Evidence map›Paper›PMID 21982778›Full record

ArticleMitochondrion2012

Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy.

Harpreet Singh, Rong Lu, Pedro Felipe Gardeazábal Rodríguez, Yong Wu, Jean Chrisostome Bopassa, Enrico Stefani, Ligia Toro

Abstract read
In one paragraph

Article in Mitochondrion, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. The BKPhysiological reports · 2024
    Article
  6. Article
  7. Article
  8. Inhibition of BKCell death discovery · 2022
    Article
  9. Article
  10. Tumor-Induced Cardiac Dysfunction: A Potential Role of ROS.Antioxidants (Basel, Switzerland) · 2021
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. BKCells · 2019
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Harpreet SinghDepartment of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Rong Lu
Pedro Felipe Gardeazábal Rodríguez
Yong Wu
Jean Chrisostome Bopassa
Enrico Stefani
Ligia Toro

Funding

Revealing Cardiovascular Stress Regulation beyond the Diffraction LimitR01HL088640 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI STEFANI, ENRICO · 2007 to 2011
$5.3M
STRUCTURAL DETERMINANTS OF K(CA) CHANNEL FUNCTIONR01HL054970 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORO DE STEFANI, LIGIA G. · 1995 to 2009
$3.2M
NHLBI NIH HHS HL088640NHLBI NIH HHS HL54970NHLBI NIH HHS R01 HL054970NHLBI NIH HHS R01 HL088640
6 · The paper itself

Abstract

The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~30nm lateral resolution for protein mapping of Percoll-purified viable mitochondria from murine heart. Using this approach, we were able to quantify and resolve distinct protein clusters within mitochondria; specifically, cytochrome c oxidase subunit 2 is distributed in clusters of ~28nm; whereas the voltage dependent anion channel 1 displays three size distributions of ~33, ~55 and ~83nm.

Indexed as

AnimalsMaleMiceMice, Inbred C57BLMicroscopy, FluorescenceMitochondriaMitochondrial ProteinsMyocardiumMitochondrial Proteins

Identifiers

PMID21982778
PMCPMC3258335

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.