ArticleJournal of neuroscience methods2012
Mannitol-facilitated perfusion staining with 2,3,5-triphenyltetrazolium chloride (TTC) for detection of experimental cerebral infarction and biochemical analysis.
Article in Journal of neuroscience methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchyma.Life science alliance · 2022Article
- Mild traumatic brain injury increases vulnerability to cerebral ischemia in mice.Experimental neurology · 2021Article
- Fibrauretine reduces ischemia/reperfusion injury via RISK/eNOS activation.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Can We Use 2,3,5-Triphenyltetrazolium Chloride-Stained Brain Slices for Other Purposes? The Application of Western Blotting.Frontiers in molecular neuroscience · 2019Article
- Altered expression of thrombospondin-1/-2 in the cortex and synaptophysin in the hippocampus after middle cerebral artery occlusion and reperfusion.International journal of clinical and experimental pathology · 2018Article
- Article
- Effects of BDNF-Transfected BMSCs on Neural Functional Recovery and Synaptophysin Expression in Rats with Cerebral Infarction.Molecular neurobiology · 2017Article
- Prospects of modeling poststroke epileptogenesis.Journal of neuroscience research · 2017Review
- Validation of an automated colony counting system for group A Streptococcus.BMC research notes · 2016Article
- Hyperlipidemia exacerbates cerebral injury through oxidative stress, inflammation and neuronal apoptosis in MCAO/reperfusion rats.Experimental brain research · 2015Article
- A Thrombotic Stroke Model Based On Transient Cerebral Hypoxia-ischemia.Journal of visualized experiments : JoVE · 2015Article
- Article
- Taming neonatal hypoxic-ischemic brain injury by intranasal delivery of plasminogen activator inhibitor-1.Stroke · 2013Article
- Intranasal delivery of cell-penetrating anti-NF-κB peptides (Tat-NBD) alleviates infection-sensitized hypoxic-ischemic brain injury.Experimental neurology · 2013Article
- Towards stratifying ischemic components by cardiac MRI and multifunctional stainings in a rabbit model of myocardial infarction.Theranostics · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A simple method to quantify cerebral infarction has great value for mechanistic and therapeutic studies in experimental stroke research. Immersion staining of unfixed brain slices with 2,3,5-triphenyltetrazolium chloride (TTC) is a popular method to determine cerebral infarction in preclinical studies. However, it is often difficult to apply immersion TTC-labeling to severely injured or soft newborn brains in rodents. Here we report an in vivo TTC perfusion-labeling method based on osmotic opening of blood-brain-barrier with mannitol-pretreatment. This new method delineates cortical infarction correlated with the boundary of morphological cell injury, differentiates the induction or subcellular redistribution of apoptosis-related factors between viable and damaged areas, and easily determines the size of cerebral infarction in both adult and newborn mice. Using this method, we confirmed that administration of lipopolysaccharide 72 h before hypoxia-ischemia increases the damage in neonatal mouse brains, in contrast to its effect of protective preconditioning in adults. These results demonstrate a fast and inexpensive method that simplifies the task of quantifying cerebral infarction in small or severely injured brains and assists biochemical analysis of experimental cerebral ischemia.
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