ArticleTransfusion2012
Alloimmunization to transfused platelets requires priming of CD4+ T cells in the splenic microenvironment in a murine model.
Article in Transfusion, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- HLA class I antibody distribution and cross-matching strategies in immune platelet transfusion refractory patients: a retrospective cohort study.Frontiers in medicine · 2026Article
- Interactions with and activation of immune cells by CD41aFrontiers in immunology · 2025Article
- Immune interactions and regulation with CD39Frontiers in immunology · 2024Article
- Marginal zone B cells are responsible for the production of alloantibodies following platelet transfusion in mice.Blood advances · 2023Article
- Polyinosinic: polycytidylic acid induced inflammation enhances while lipopolysaccharide diminishes alloimmunity to platelet transfusion in mice.Frontiers in immunology · 2023Article
- Divergent CD4Frontiers in immunology · 2023Article
- CD27Frontiers in immunology · 2023Article
- Current Status of and Global Trends in Platelet Transfusion Refractoriness From 2004 to 2021: A Bibliometric Analysis.Frontiers in medicine · 2022Article
- Anti-JMH alloantibody in inherited JMH-negative patients leads to immunogenic destruction of JMH-positive RBCs.Clinical and experimental immunology · 2021Article
- Transfusion-induced platelet antibodies and regulatory T cells in multiply transfused patients.Journal of clinical laboratory analysis · 2021Article
- Characterization of glycoprotein IIb/IIIa-specific alloantibodies induced by cross-strain platelet immunization in mice.Transfusion · 2021Article
- In utero exposure to alloantigens primes alloimmunization to platelet transfusion in mice.Transfusion · 2021Article
- Pathogen-reduced PRP blocks T-cell activation, induces Treg cells, and promotes TGF-β expression by cDCs and monocytes in mice.Blood advances · 2020Article
- Recapitulation of posttransfusion purpura by cross-strain platelet immunization in mice.Blood advances · 2020Article
- Transfused platelets enhance alloimmune responses to transfused KEL-expressing red blood cells in a murine model.Blood transfusion = Trasfusione del sangue · 2019Article
- Immunological Features in the Process of Blood Platelet-Induced Alloimmunisation, with a Focus on Platelet Component Transfusion.Diseases (Basel, Switzerland) · 2019Review
- Storage-Induced Platelet Apoptosis Is a Potential Risk Factor for Alloimmunization Upon Platelet Transfusion.Frontiers in immunology · 2018Article
- Type I IFN Is Necessary and Sufficient for Inflammation-Induced Red Blood Cell Alloimmunization in Mice.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Absence of the spleen and the occurrence of primary red cell alloimmunization in humans.Haematologica · 2017Article
- CD4 Depletion or CD40L Blockade Results in Antigen-Specific Tolerance in a Red Blood Cell Alloimmunization Model.Frontiers in immunology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlloantibodies are a clinically significant sequelae of platelet (PLT) transfusion, potentially rendering patients refractory to ongoing PLT transfusion support. These antibodies are often IgG class switched, suggesting the involvement of CD4+ T-cell help; however, PLT-specific CD4+ T cells have not been visualized in vivo, and specifics of their stimulation are not completely understood. STUDY DESIGN AND
methodsA murine model of alloimmunization to transfused PLTs was developed to allow in vivo assessment and characterization of CD4+ T cells specific for PLT major histocompatibility complex (MHC) alloantigen. PLTs were harvested from BALB/c mice, filter leukoreduced, and transfused into C57BL/6 recipients. PLT-specific CD4+ T-cell responses were visualized by using a T-cell receptor transgenic mouse that detects peptide from donor MHC I presented on recipient MHC II. Antibody responses were determined by indirect immunofluorescence using BALB/c donor targets.
resultsC57BL/6 recipients of BALB/c leukoreduced PLT transfusions produced BALB/c antibodies, with proliferation of antigen-specific CD4+ T cells seen in the spleen but not lymph nodes or liver. Depletion of recipient CD4+ cells or splenectomy independently abrogated the alloantibody response.
conclusionWe report a novel model to study antigen-specific CD4+ T cells during alloimmunization to PLT transfusion. The presented data support a critical role for CD4+ T-cell help in the humoral response to PLT transfusion and establish the spleen as a required microenvironment for effective CD4+ T-cell priming against donor PLT-derived MHC I.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.