ArticleTraffic (Copenhagen, Denmark)2012
Post-Golgi supramolecular assembly of aquaporin-4 in orthogonal arrays.
Article in Traffic (Copenhagen, Denmark), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- In Silico Modelling of the AQP0 T138R Mutation and its' links to Potential Mechanisms of Cataractogenesis.Research square · 2025Article
- Aquaporin-4 in Neuromyelitis Optica Spectrum Disorders: A Target of Autoimmunity in the Central Nervous System.Biomolecules · 2022Review
- Review
- Article
- A new M23-based ELISA assay for anti-aquaporin 4 autoantibodies: diagnostic accuracy and clinical correlation.Auto- immunity highlights · 2019Article
- Article
- Comparative molecular dynamics study of neuromyelitis optica-immunoglobulin G binding to aquaporin-4 extracellular domains.Biochimica et biophysica acta. Biomembranes · 2017Article
- Human Aquaporin-4 and Molecular Modeling: Historical Perspective and View to the Future.International journal of molecular sciences · 2016Review
- Astrocyte Aquaporin Dynamics in Health and Disease.International journal of molecular sciences · 2016Review
- Experimental Evaluation of Proposed Small-Molecule Inhibitors of Water Channel Aquaporin-1.Molecular pharmacology · 2016Article
- Transient hyperckemia in the setting of neuromyelitis optica (NMO).Muscle & nerve · 2014Article
- Identification of a point mutation impairing the binding between aquaporin-4 and neuromyelitis optica autoantibodies.The Journal of biological chemistry · 2014Article
- Evaluation of aquaporin-4 antibody assays.Clinical & experimental neuroimmunology · 2014Article
- Aggregation state determines the localization and function of M1- and M23-aquaporin-4 in astrocytes.The Journal of cell biology · 2014Article
- Biology of AQP4 and anti-AQP4 antibody: therapeutic implications for NMO.Brain pathology (Zurich, Switzerland) · 2013Review
- Physiological roles of aquaporin-4 in brain.Physiological reviews · 2013Review
- Rab6a/a' are important Golgi regulators of pro-inflammatory TNF secretion in macrophages.PloS one · 2013Article
- Article
- Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes.Journal of cell science · 2012Article
- Complement-dependent cytotoxicity in neuromyelitis optica requires aquaporin-4 protein assembly in orthogonal arrays.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The supramolecular assembly of aquaporin-4 (AQP4) in orthogonal arrays of particles (OAPs) involves N-terminus interactions of the M23-AQP4 isoform. We found AQP4 OAPs in cell plasma membranes but not in endoplasmic reticulum (ER) or Golgi, as shown by: (i) native gel electrophoresis of brain and AQP4-transfected cells, (ii) photobleaching recovery of green fluorescent protein-AQP4 chimeras in live cells and (iii) freeze-fracture electron microscopy (FFEM). We found that AQP4 OAP formation in plasma membranes, but not in the Golgi, was not related to AQP4 density, pH, membrane lipid composition, C-terminal PDZ domain interactions or α-syntrophin expression. Remarkably, however, fusion of AQP4-containing Golgi vesicles with (AQP4-free) plasma membrane vesicles produced OAPs, suggesting the involvement of plasma membrane factor(s) in AQP4 OAP formation. In investigating additional possible determinants of OAP assembly we discovered membrane curvature-dependent OAP assembly, in which OAPs were disrupted by extrusion of plasma membrane vesicles to ∼110 nm diameter, but not to ∼220 nm diameter. We conclude that AQP4 supramolecular assembly in OAPs is a post-Golgi phenomenon involving plasma membrane-specific factor(s). Post-Golgi and membrane curvature-dependent OAP assembly may be important for vesicle transport of AQP4 in the secretory pathway and AQP4-facilitated astrocyte migration, and suggests a novel therapeutic approach for neuromyelitis optica.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.