ArticleMolecular plant pathology2012
RNA-mediated gene silencing of ToxB in Pyrenophora tritici-repentis.
Article in Molecular plant pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 33 citations in OpenAlex.
- An optimized sporulation method for the wheat fungal pathogen Pyrenophora tritici-repentis.Plant methods · 2021Article
- Enniatin Production InfluencesPathogens (Basel, Switzerland) · 2020Article
- Identification of a Locus Conferring Dominant Susceptibility toFrontiers in plant science · 2020Article
- Comparative genome analysis indicates high evolutionary potential of pathogenicity genes in Colletotrichum tanaceti.PloS one · 2019Article
- Necrotrophic effector epistasis in the Pyrenophora tritici-repentis-wheat interaction.PloS one · 2015Article
- Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis.Molecular plant pathology · 2014Article
- Investigation of the Fusarium virguliforme fvtox1 mutants revealed that the FvTox1 toxin is involved in foliar sudden death syndrome development in soybean.Current genetics · 2013Article
- Comparative genomics of a plant-pathogenic fungus, Pyrenophora tritici-repentis, reveals transduplication and the impact of repeat elements on pathogenicity and population divergence.G3 (Bethesda, Md.) · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fungus Pyrenophora tritici-repentis causes tan spot, a wheat leaf disease of worldwide importance. The pathogen produces three host-selective toxins, including Ptr ToxB, which causes chlorophyll degradation and foliar chlorosis on toxin-sensitive wheat genotypes. The ToxB gene, which codes for Ptr ToxB, was silenced in a wild-type race 5 isolate of the fungus thorough a sense- and antisense-mediated silencing mechanism. Toxin production by the silenced strains was evaluated in culture filtrates of the fungus via Western blotting analysis, and plant bioassays were conducted to test the virulence of the transformants in planta. The chlorosis-inducing ability of the silenced strains was correlated with the quantity of Ptr ToxB, and transformants in which toxin production was strongly decreased also caused very little disease on toxin-sensitive wheat genotypes. Cytological analysis of the infection process revealed that, in addition to a reduced capacity to induce chlorosis, the silenced strains with the greatest decrease in the levels of Ptr ToxB produced significantly fewer appressoria than the wild-type isolate, 12 and 24 h after inoculation onto wheat leaves. The results provide strong support for the suggestion that the amount of Ptr ToxB protein produced by fungal isolates plays a significant role in the quantitative variation in the virulence of P. tritici-repentis.
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