ArticlePloS one2011
Lateral diffusion on tubular membranes: quantification of measurements bias.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
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- Sub-membrane actin rings compartmentalize the plasma membrane.The Journal of cell biology · 2024Article
- Non-Markovian Diffusion and Adsorption-Desorption Dynamics: Analytical and Numerical Results.Entropy (Basel, Switzerland) · 2024Article
- Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- A local interplay between diffusion and intraflagellar transport distributes TRPV-channel OCR-2 along C. elegans chemosensory cilia.Communications biology · 2022Article
- Single molecule tracking of bacterial cell surface cytochromes reveals dynamics that impact long-distance electron transport.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- High-Resolution Fluorescence Imaging Combined With Computer Simulations to Quantitate Surface Dynamics and Nanoscale Organization of Neuroligin-1 at Synapses.Frontiers in synaptic neuroscience · 2022Article
- Modeling of the axon plasma membrane structure and its effects on protein diffusion.PLoS computational biology · 2019Article
- Motion in a crowded environment: the influence of obstacles' size and shape and model of transport.Journal of molecular modeling · 2019Article
- Inhibitory Receptor Diffusion Dynamics.Frontiers in molecular neuroscience · 2019Article
- Single-particle trajectories reveal two-state diffusion-kinetics of hOGG1 proteins on DNA.Nucleic acids research · 2018Article
- A Simple and Powerful Analysis of Lateral Subdiffusion Using Single Particle Tracking.Biophysical journal · 2017Article
- Article
- Dynamic imaging of mitochondrial membrane proteins in specific sub-organelle membrane locations.Biophysical reviews · 2017Review
- Analysis of diffusion in curved surfaces and its application to tubular membranes.Molecular biology of the cell · 2016Article
- Carbon Nanotube Based Groundwater Remediation: The Case of Trichloroethylene.Molecules (Basel, Switzerland) · 2016Article
- Targeting neurotransmitter receptors with nanoparticles in vivo allows single-molecule tracking in acute brain slices.Nature communications · 2016Article
- Article
- Fast Three-Dimensional Single-Particle Tracking in Natural Brain Tissue.Biophysical journal · 2015Article
- Multiple spatial and kinetic subpopulations of CaMKII in spines and dendrites as resolved by single-molecule tracking PALM.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single Particle Tracking (SPT) is a powerful technique for the analysis of the lateral diffusion of the lipid and protein components of biological membranes. In neurons, SPT allows the study of the real-time dynamics of receptors for neurotransmitters that diffuse continuously in and out synapses. In the simplest case where the membrane is flat and is parallel to the focal plane of the microscope the analysis of diffusion from SPT data is relatively straightforward. However, in most biological samples the membranes are curved, which complicates analysis and may lead to erroneous conclusions as for the mode of lateral diffusion. Here we considered the case of lateral diffusion in tubular membranes, such as axons, dendrites or the neck of dendritic spines. Monte Carlo simulations allowed us to evaluate the error in diffusion coefficient (D) calculation if the curvature is not taken into account. The underestimation is determined by the diameter of the tubular surface, the frequency of image acquisition and the degree of mobility itself. We found that projected trajectories give estimates that are 25 to 50% lower than the real D in case of 2D-SPT over the tubular surface. The use of 3D-SPT improved the measurements if the frequency of image acquisition was fast enough in relation to the mobility of the molecules and the diameter of the tube. Nevertheless, the calculation of D from the components of displacements in the axis of the tubular structure gave accurate estimate of D, free of geometrical artefacts. We show the application of this approach to analyze the diffusion of a lipid on model tubular membranes and of a membrane-bound GFP on neurites from cultured rat hippocampal neurons.
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