ArticleJournal of lipid research2011
The polypyrimidine tract binding protein regulates desaturase alternative splicing and PUFA composition.
Article in Journal of lipid research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Alternative Splicing Regulation in Metabolic Disorders.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2025Review
- Altered splicing factor and alternative splicing events in a mouse model of diet- and polychlorinated biphenyl-induced liver disease.Environmental toxicology and pharmacology · 2023Article
- Targeting microRNA-145-mediated progressive phenotypes of early bladder cancer in a molecularly definedMolecular therapy. Nucleic acids · 2023Article
- Dysregulation of the Splicing Machinery Is Associated to the Development of Nonalcoholic Fatty Liver Disease.The Journal of clinical endocrinology and metabolism · 2019Article
- A novel FADS2 isoform identified in human milk fat globule suppresses FADS2 mediated Δ6-desaturation of omega-3 fatty acids.Prostaglandins, leukotrienes, and essential fatty acids · 2018Article
- PTBP1 acts as a dominant repressor of the aberrant tissue-specific splicing of ISCU in hereditary myopathy with lactic acidosis.Molecular genetics & genomic medicine · 2018Article
- The emerging role of alternative splicing in senescence and aging.Aging cell · 2017Review
- Fads3 modulates docosahexaenoic acid in liver and brain.Prostaglandins, leukotrienes, and essential fatty acids · 2017Article
- Alternative RNA Splicing in the Pathogenesis of Liver Disease.Frontiers in endocrinology · 2017Review
- Desaturase and elongase-limiting endogenous long-chain polyunsaturated fatty acid biosynthesis.Current opinion in clinical nutrition and metabolic care · 2016Review
- Role of precursor mRNA splicing in nutrient-induced alterations in gene expression and metabolism.The Journal of nutrition · 2015Review
- HNRNPA1 regulates HMGCR alternative splicing and modulates cellular cholesterol metabolism.Human molecular genetics · 2014Article
- Regulation of alternative splicing in obesity and weight loss.Adipocyte · 2013Article
- Alternative splicing in the regulation of cholesterol homeostasis.Current opinion in lipidology · 2013Review
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The Δ6 desaturase, encoded by FADS2, plays a crucial role in omega-3 and omega-6 fatty acid synthesis. These fatty acids are essential components of the central nervous system, and they act as precursors for eicosanoid signaling molecules and as direct modulators of gene expression. The polypyrimidine tract binding protein (PTB or hnRNP I) is a splicing factor that regulates alternative pre-mRNA splicing. Here, PTB is shown to bind an exonic splicing silencer element and repress alternative splicing of FADS2 into FADS2 AT1. PTB and FADS2AT1 were inversely correlated in neonatal baboon tissues, implicating PTB as a major regulator of tissue-specific FADS2 splicing. In HepG2 cells, PTB knockdown modulated alternative splicing of FADS2, as well as FADS3, a putative desaturase of unknown function. Omega-3 fatty acids decreased by nearly one half relative to omega-6 fatty acids in PTB knockdown cells compared with controls, with a particularly strong decrease in eicosapentaenoic acid (EPA) concentration and its ratio to arachidonic acid (ARA). This is a rare demonstration of a mechanism specifically altering the cellular omega-3 to omega-6 fatty acid ratio without any change in diet/media. These findings reveal a novel role for PTB, regulating availability of membrane components and eicosanoid precursors for cell signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.