ArticleBMC systems biology2011
Genomic phenotyping of the essential and non-essential yeast genome detects novel pathways for alkylation resistance.
Article in BMC systems biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- One Yeast, Sixteen Synthetic Chromosomes, Infinite Possibilities.Yeast (Chichester, England) · 2026Review
- The putative error prone polymerasenpj antimicrobials and resistance · 2024Article
- A multiplexed, three-dimensional pooling and next-generation sequencing strategy for creating barcoded mutant arrays: construction of a Schizosaccharomyces pombe transposon insertion library.Nucleic acids research · 2022Article
- Synthetic refactor of essential genes decodes functionally constrained sequences in yeast genome.iScience · 2022Article
- Loss of Mediator complex subunit 13 (MED13) promotes resistance to alkylation through cyclin D1 upregulation.Nucleic acids research · 2021Article
- Hof1 plays a checkpoint-related role in MMS-induced DNA damage response inMolecular biology of the cell · 2020Article
- Nucleotide Excision Repair Protein Rad23 Regulates Cell Virulence Independent of Rad4 in Candida albicans.mSphere · 2020Article
- Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress.The Journal of cell biology · 2017Article
- Quality control of chemically damaged RNA.Cellular and molecular life sciences : CMLS · 2016Review
- Tryptophan biosynthesis is important for resistance to replicative stress in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2016Article
- Mutant power: using mutant allele collections for yeast functional genomics.Briefings in functional genomics · 2016Review
- Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells.DNA repair · 2014Article
- A Nudix Hydrolase Protein, Ysa1, Regulates Oxidative Stress Response and Antifungal Drug Susceptibility in Cryptococcus neoformans.Mycobiology · 2014Article
- Article
- Genome-wide single-cell-level screen for protein abundance and localization changes in response to DNA damage in S. cerevisiae.Nucleic acids research · 2013Article
- Article
- Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.BMC genomics · 2012Article
- Identification of novel human damage response proteins targeted through yeast orthology.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
Abstract
backgroundA myriad of new chemicals has been introduced into our environment and exposure to these agents can damage cells and induce cytotoxicity through different mechanisms, including damaging DNA directly. Analysis of global transcriptional and phenotypic responses in the yeast S. cerevisiae provides means to identify pathways of damage recovery upon toxic exposure.
resultsHere we present a phenotypic screen of S. cerevisiae in liquid culture in a microtiter format. Detailed growth measurements were analyzed to reveal effects on ~5,500 different haploid strains that have either non-essential genes deleted or essential genes modified to generate unstable transcripts. The pattern of yeast mutants that are growth-inhibited (compared to WT cells) reveals the mechanisms ordinarily used to recover after damage. In addition to identifying previously-described DNA repair and cell cycle checkpoint deficient strains, we also identified new functional groups that profoundly affect MMS sensitivity, including RNA processing and telomere maintenance.
conclusionsWe present here a data-driven method to reveal modes of toxicity of different agents that impair cellular growth. The results from this study complement previous genomic phenotyping studies as we have expanded the data to include essential genes and to provide detailed mutant growth analysis for each individual strain. This eukaryotic testing system could potentially be used to screen compounds for toxicity, to identify mechanisms of toxicity, and to reduce the need for animal testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.