ArticleVirology journal2011
Establishing a TaqMan-based real-time PCR assay for the rapid detection and quantification of the newly emerged duck Tembusu virus.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 62 citations in OpenAlex.
- Transmission routes of cluster 3 Tembusu virus in ducks and chickens.The Journal of general virology · 2025Article
- Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome.Veterinary sciences · 2025Article
- Comparative analysis of duck Tembusu virus Cluster 1 and Cluster 2.1 inCurrent research in parasitology & vector-borne diseases · 2025Article
- Article
- Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.Animals : an open access journal from MDPI · 2024Article
- Pathogenicity and Interspecies Transmission of Cluster 3 Tembusu Virus Strain TMUV HQ-22 Isolated from Geese.Viruses · 2023Article
- 34-kDa salivary protein enhances duck Tembusu virus infectivity in the salivary glands of Aedes albopictus by modulating the innate immune response.Scientific reports · 2023Article
- Development of a reverse transcription loop-mediated isothermal amplification based clustered regularly interspaced short palindromic repeats Cas12a assay for duck Tembusu virus.Frontiers in microbiology · 2023Article
- Duck Tembusu Virus Inhibits Type I Interferon Production through the JOSD1-SOCS1-IRF7 Negative-Feedback Regulation Pathway.Journal of virology · 2022Article
- Rapid detection of H146-like goose calicivirus using a TaqMan-based real-time PCR assay.Poultry science · 2021Article
- Determinants of duck Tembusu virus NS2A/2B polyprotein procession attenuated viral replication and proliferation in vitro.Scientific reports · 2020Article
- Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus.BMC veterinary research · 2020Article
- The Emerging Duck Flavivirus Is Not Pathogenic for Primates and Is Highly Sensitive to Mammalian Interferon Antiviral Signaling.Journal of virology · 2016Article
- Advanced uracil DNA glycosylase-supplemented real-time reverse transcription loop-mediated isothermal amplification (UDG-rRT-LAMP) method for universal and specific detection of Tembusu virus.Scientific reports · 2016Article
- Development of a PCR-Based Reverse Genetics System for an Attenuated Duck Tembusu Virus Strain.PloS one · 2016Article
- Airborne Transmission of a Novel Tembusu Virus in Ducks.Journal of clinical microbiology · 2015Article
- Development of conventional and real-time reverse transcription polymerase chain reaction assays to detect Tembusu virus in Culex tarsalis mosquitoes.The American journal of tropical medicine and hygiene · 2014Article
- Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP).Virology journal · 2014Article
- Adaptation and attenuation of duck Tembusu virus strain Du/CH/LSD/110128 following serial passage in chicken embryos.Clinical and vaccine immunology : CVI · 2014Article
- Reverse transcription loop-mediated isothermal amplification for rapid detection of the newly emerged poultry Flavivirus.Folia microbiologica · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To establish an accurate, rapid, and a quantifiable method for the detection of the newly emerged duck Tembusu virus (DTMUV) that recently caused a widespread infectious disease in ducks in China, we developed a TaqMan-based real-time PCR assay by using E gene-specific primers and a TaqMan probe. This real-time PCR assay was 100 times more sensitive than the conventional PCR. The reproducibility and specificity of the real-time PCR assay were confirmed using plasmids containing E genes or RNAs and DNAs extracted from well-known viruses causing duck diseases. The reliability of this real-time PCR assay was confirmed in 19 of the 24 swab samples, 22 of the 24 tissue samples collected from experimentally infected ducks, as well as 15 of the 21 clinical samples collected from sick ducks since they were verified as DTMUV-positive. The results reveal that the newly established real-time PCR assay might be a useful diagnostic method for epidemiologically investigating and closely observing the newly emerged DTMUV.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.