ArticleCell calcium2011
Imaging of a glucose analog, calcium and NADH in neurons and astrocytes: dynamic responses to depolarization and sensitivity to pioglitazone.
Article in Cell calcium, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Central cytometabolic functional vascular coupling in health and disease.npj metabolic health and disease · 2025Review
- Variable bioenergetic sensitivity of neurons and astrocytes to insulin and extracellular glucose.npj metabolic health and disease · 2024Article
- Extracellular levels of glucose in the hippocampus and striatum during maze training for food or water reward in male rats.Behavioural brain research · 2021Article
- Single-Cell Microwell Platform Reveals Circulating Neural Cells as a Clinical Indicator for Patients with Blood-Brain Barrier Breakdown.Research (Washington, D.C.) · 2021Article
- L-Glucose: Another Path to Cancer Cells.Cancers · 2020Review
- Elevating Insulin Signaling Using a Constitutively Active Insulin Receptor Increases Glucose Metabolism and Expression of GLUT3 in Hippocampal Neurons.Frontiers in neuroscience · 2020Article
- Systems biology identifies preserved integrity but impaired metabolism of mitochondria due to a glycolytic defect in Alzheimer's disease neurons.Aging cell · 2019Article
- Broadening the definition of brain insulin resistance in aging and Alzheimer's disease.Experimental neurology · 2019Review
- Expression of a Constitutively Active Human Insulin Receptor in Hippocampal Neurons Does Not Alter VGCC Currents.Neurochemical research · 2019Article
- Novel calcium-related targets of insulin in hippocampal neurons.Neuroscience · 2017Article
- Insulin Resistance and Alzheimer's Disease: Bioenergetic Linkages.Frontiers in aging neuroscience · 2017Review
- Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism.Nature communications · 2015Article
- Pioglitazone rapidly reduces neuropathic pain through astrocyte and nongenomic PPARγ mechanisms.Pain · 2015Article
- Hippocampal calcium dysregulation at the nexus of diabetes and brain aging.European journal of pharmacology · 2013Review
- The role of astrocytic glycogen in supporting the energetics of neuronal activity.Neurochemical research · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Neuronal Ca(2+) dyshomeostasis associated with cognitive impairment and mediated by changes in several Ca(2+) sources has been seen in animal models of both aging and diabetes. In the periphery, dysregulation of intracellular Ca(2+) signals may contribute to the development of insulin resistance. In the brain, while it is well-established that type 2 diabetes mellitus is a risk factor for the development of dementia in the elderly, it is not clear whether Ca(2+) dysregulation might also affect insulin sensitivity and glucose utilization. Here we present a combination of imaging techniques testing the disappearance of the fluorescent glucose analog 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) as an indication of glycolytic activity in neurons and astrocytes. Our work shows that glucose utilization at rest is greater in neurons compared to astrocytes, and ceases upon activation in neurons with little change in astrocytes. Pretreatment of hippocampal cultures with pioglitazone, a drug used in the treatment of type 2 diabetes, significantly reduced glycolytic activity in neurons and enhanced it in astrocytes. This series of experiments, including Fura-2 and NADH imaging, provides results that are consistent with the idea that Ca(2+) levels may rapidly alter glycolytic activity, and that downstream events beyond Ca(2+) dysregulation with aging, may alter cellular metabolism in the brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.