ArticleJournal of clinical microbiology2011
Molecular detection and species-specific identification of medically important Aspergillus species by real-time PCR in experimental invasive pulmonary aspergillosis.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 85 citations in OpenAlex.
- Development and Validation of a Real-Time PCR Assay for the Detection of Aspergillus fumigatus on Panther Fusion System.Methods in molecular biology (Clifton, N.J.) · 2026Article
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- A Laboratory-Developed Assay for the Simultaneous Detection ofJournal of fungi (Basel, Switzerland) · 2025Article
- Mycobiome analyses of critically ill COVID-19 patients.Microbiology spectrum · 2025Article
- Development of a Multiplex Real-Time PCR Assay for the Simultaneous Detection of Two Fungal Pathogens Causing Pneumonia.Journal of fungi (Basel, Switzerland) · 2024Article
- Development of a novel mycobiome diagnostic for fungal infection.BMC microbiology · 2024Article
- Identification of closely related species inFrontiers in microbiology · 2024Article
- The Deciphering of Growth-Dependent Strategies for Quorum-Sensing Networks inMicroorganisms · 2023Article
- Attention-Guided Transfer Learning for Identification of Filamentous Fungi Encountered in the Clinical Laboratory.Microbiology spectrum · 2023Article
- Mortality Causes in Captive Cantabrian capercaillie (Animals : an open access journal from MDPI · 2023Article
- Invasive Pulmonary Aspergillosis.Journal of fungi (Basel, Switzerland) · 2023Review
- Detection and identification ofFrontiers in cellular and infection microbiology · 2023Article
- Phenotypic Variants of Azole-Resistant Aspergillus Fumigatus that Co-exist in Human Respiratory Samples are Genetically Highly Related.Mycopathologia · 2022Article
- Molecular identification ofJournal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 2022Article
- Modeling Invasive Aspergillosis: How Close Are Predicted Antifungal Targets?Journal of fungi (Basel, Switzerland) · 2020Review
- Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification ofInternational journal of molecular sciences · 2020Article
- Multiplex PCR of bronchoalveolar lavage fluid in children enhances the rate of pathogen detection.BMC pulmonary medicine · 2019Article
- Invasive Aspergillosis byJournal of fungi (Basel, Switzerland) · 2019Review
- Pulmonary Metagenomic Sequencing Suggests Missed Infections in Immunocompromised Children.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present.Microorganisms · 2019Review
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diagnosis of invasive pulmonary aspergillosis (IPA) remains a major challenge to clinical microbiology laboratories. We developed rapid and sensitive quantitative PCR (qPCR) assays for genus- and species-specific identification of Aspergillus infections by use of TaqMan technology. In order to validate these assays and understand their potential diagnostic utility, we then performed a blinded study of bronchoalveolar lavage (BAL) fluid specimens from well-characterized models of IPA with the four medically important species. A set of real-time qPCR primers and probes was developed by utilizing unique ITS1 regions for genus- and species-specific detection of the four most common medically important Aspergillus species (Aspergillus fumigatus, A. flavus, A. niger, and A. terreus). Pan-Aspergillus and species-specific qPCRs with BAL fluid were more sensitive than culture for detection of IPA caused by A. fumigatus in untreated (P < 0.0007) and treated (P ≤ 0.008) animals, respectively. For infections caused by A. terreus and A. niger, culture and PCR amplification from BAL fluid yielded similar sensitivities for untreated and treated animals. Pan-Aspergillus PCR was more sensitive than culture for detection of A. flavus in treated animals (P = 0.002). BAL fluid pan-Aspergillus and species-specific PCRs were comparable in sensitivity to BAL fluid galactomannan (GM) assay. The copy numbers from the qPCR assays correlated with quantitative cultures to determine the pulmonary residual fungal burdens in lung tissue. Pan-Aspergillus and species-specific qPCR assays may improve the rapid and accurate identification of IPA in immunocompromised patients.
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