ArticleNucleic acids research2011
Label-free high-throughput microRNA expression profiling from total RNA.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- A selective enrichment and specific probe terminal mediated strategy for highly sensitive detection of microRNAs.Nature communications · 2026Article
- Overview of exosomal non-coding RNAs in cardiovascular disease using high throughput sequencing.European journal of pharmacology · 2025Review
- A dual-mode biosensor for microRNA detection based on DNA tetrahedron-gated nanochannels.Mikrochimica acta · 2025Article
- Integrated Profiling of Extracellular Vesicle microRNA Impact on Trabecular Meshwork mRNA Expression: Insights From Microarray Analysis.Investigative ophthalmology & visual science · 2024Article
- Rapid in situ RNA imaging based on Cas12a thrusting strand displacement reaction.Nucleic acids research · 2023Article
- New discoveries in the field of metabolism by applying single-cell and spatial omics.Journal of pharmaceutical analysis · 2023Review
- Single cell quantification of microRNA from small numbers of non-invasively sampled primary human cells.Communications biology · 2023Article
- Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis.Acta pharmaceutica Sinica. B · 2023Review
- "Silicon-On-Insulator"-Based Biosensor for the Detection of MicroRNA Markers of Ovarian Cancer.Micromachines · 2022Article
- miRNA in Molecular Diagnostics.Bioengineering (Basel, Switzerland) · 2022Review
- DNA Transformations for Diagnosis and Therapy.Advanced functional materials · 2021Review
- Integrating CRISPR-Cas12a with a DNA circuit as a generic sensing platform for amplified detection of microRNA.Chemical science · 2020Article
- Preparation of antibody-immobilized gelatin nanospheres incorporating a molecular beacon to visualize the biological function of macrophages.Regenerative therapy · 2020Article
- A luminescent microRNA nanoprobe based on the target-triggered release of an iridium(III)-solvent complex from mesoporous silica nanoparticles.Mikrochimica acta · 2019Article
- DNA nanotechnology approaches for microRNA detection and diagnosis.Nucleic acids research · 2019Review
- Differentially Expressed microRNAs in MIA PaCa-2 and PANC-1 Pancreas Ductal Adenocarcinoma Cell Lines are Involved in Cancer Stem Cell Regulation.International journal of molecular sciences · 2019Article
- Clinical value of microRNA-198-5p downregulation in lung adenocarcinoma and its potential pathways.Oncology letters · 2019Article
- Genome-wide study of salivary microRNAs as potential noninvasive biomarkers for detection of nasopharyngeal carcinoma.BMC cancer · 2019Article
- MicroRNA amplification and detection technologies: opportunities and challenges for point of care diagnostics.Laboratory investigation; a journal of technical methods and pathology · 2019Review
- Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are key biological regulators and promising disease markers whose detection technologies hold great potentials in advancing fundamental research and medical diagnostics. Currently, miRNAs in biological samples have to be labeled before being applied to most high-throughput assays. Although effective, these labeling-based approaches are usually labor-intensive, time-consuming and liable to bias. Besides, the cross-hybridization of co-existing miRNA precursors (pre-miRNAs) is not adequately addressed in most assays that use total RNA as input. Here, we present a hybridization-triggered fluorescence strategy for label-free, microarray-based high-throughput miRNA expression profiling. The total RNA is directly applied to the microarray with a short fluorophore-linked oligonucleotide Universal Tag which can be selectively captured by the target-bound probes via base-stacking effects. This Stacking-Hybridized Universal Tag (SHUT) assay has been successfully used to analyze as little as 100 ng total RNA from human tissues, and found to be highly specific to homogenous miRNAs. Superb discrimination toward single-base mismatch at the 5' or 3' end has been demonstrated. Importantly, the pre-miRNAs generated negligible signals, validating the direct use of total RNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.