ArticleNucleic acids research2012
Identification of essential and non-essential single-stranded DNA-binding proteins in a model archaeal organism.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- The canonical single-stranded DNA-binding protein is not an essential replication factor but an RNA chaperon iniScience · 2023Article
- Magnetic Nanoparticles for Protein Separation and Purification.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Single-Stranded DNA-Binding Proteins in the Archaea.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Remote Homology Detection Identifies a Eukaryotic RPA DBD-C-like DNA Binding Domain as a Conserved Feature of Archaeal Rpa1-Like Proteins.Frontiers in molecular biosciences · 2021Article
- Review
- Cyclic nucleotides in archaea: Cyclic di-AMP in the archaeon Haloferax volcanii and its putative role.MicrobiologyOpen · 2019Article
- Snapshots of archaeal DNA replication and repair in living cells using super-resolution imaging.Nucleic acids research · 2018Article
- Transcriptional Landscape and Regulatory Roles of Small Noncoding RNAs in the Oxidative Stress Response of the Haloarchaeon Haloferax volcanii.Journal of bacteriology · 2018Article
- Article
- Insights into the evolutionary conserved regulation of Rio ATPase activity.Nucleic acids research · 2018Article
- Bioengineering radioresistance by overproduction of RPA, a mammalian-type single-stranded DNA-binding protein, in a halophilic archaeon.Applied microbiology and biotechnology · 2014Article
- Identification of single-stranded and double-stranded DNA binding proteins based on protein structure.BMC bioinformatics · 2014Article
- Global phylogenomic analysis disentangles the complex evolutionary history of DNA replication in archaea.Genome biology and evolution · 2014Article
- The haloarchaeal MCM proteins: bioinformatic analysis and targeted mutagenesis of the β7-β8 and β9-β10 hairpin loops and conserved zinc binding domain cysteines.Frontiers in microbiology · 2014Article
- Polyploidy in haloarchaea: advantages for growth and survival.Frontiers in microbiology · 2014Review
- Characterization of two naturally truncated, Ssb-like proteins from the nitrogen-fixing cyanobacterium, Anabaena sp. PCC7120.Photosynthesis research · 2013Article
- Complete nucleotide sequence of Bacillus subtilis (natto) bacteriophage PM1, a phage associated with disruption of food production.Virus genes · 2013Article
- Effects of intracellular Mn on the radiation resistance of the halophilic archaeon Halobacterium salinarum.Extremophiles : life under extreme conditions · 2013Article
- Article
- The essential role of the Deinococcus radiodurans ssb gene in cell survival and radiation tolerance.PloS one · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-stranded DNA-binding proteins (SSBs) play vital roles in all aspects of DNA metabolism in all three domains of life and are characterized by the presence of one or more OB fold ssDNA-binding domains. Here, using the genetically tractable euryarchaeon Haloferax volcanii as a model, we present the first genetic analysis of SSB function in the archaea. We show that genes encoding the OB fold and zinc finger-containing RpaA1 and RpaB1 proteins are individually non-essential for cell viability but share an essential function, whereas the gene encoding the triple OB fold RpaC protein is essential. Loss of RpaC function can however be rescued by elevated expression of RpaB, indicative of functional overlap between the two classes of haloarchaeal SSB. Deletion analysis is used to demonstrate important roles for individual OB folds in RpaC and to show that conserved N- and C-terminal domains are required for efficient repair of DNA damage. Consistent with a role for RpaC in DNA repair, elevated expression of this protein leads to enhanced resistance to DNA damage. Taken together, our results offer important insights into archaeal SSB function and establish the haloarchaea as a valuable model for further studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.