ArticleThe Journal of biological chemistry2011
Uncoupling of Pyrin-only protein 2 (POP2)-mediated dual regulation of NF-κB and the inflammasome.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- An overview of human inflammasomes: Activation and regulation.Journal of immunology (Baltimore, Md. : 1950) · 2026Review
- Polydatin: A natural compound with multifaceted anticancer properties.Journal of traditional and complementary medicine · 2025Review
- New Insights on NLRP3 Inflammasome: Mechanisms of Activation, Inhibition, and Epigenetic Regulation.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2024Review
- POP1 inhibits MSU-induced inflammasome activation and ameliorates gout.Frontiers in immunology · 2022Article
- Review
- The ASC Speck and NLRP3 Inflammasome Function Are Spatially and Temporally Distinct.Frontiers in immunology · 2021Article
- An Update on CARD Only Proteins (COPs) and PYD Only Proteins (POPs) as Inflammasome Regulators.International journal of molecular sciences · 2020Review
- RNA sequencing analysis reveals apoptosis induction by hydrogen treatment in endometrial cancer via TNF and NF-κB pathways.Translational cancer research · 2020Article
- NLRP3 Sensing of Diverse Inflammatory Stimuli Requires Distinct Structural Features.Frontiers in immunology · 2020Article
- COPs and POPs Patrol Inflammasome Activation.Journal of molecular biology · 2018Review
- The PYRIN domain-only protein POP2 inhibits inflammasome priming and activation.Nature communications · 2017Article
- Pyrin-only protein 2 limits inflammation but improves protection against bacteria.Nature communications · 2017Article
- Assembly and regulation of ASC specks.Cellular and molecular life sciences : CMLS · 2017Review
- Inflammasomes and its importance in viral infections.Immunologic research · 2016Review
- Evasion and interference: intracellular pathogens modulate caspase-dependent inflammatory responses.Nature reviews. Microbiology · 2016Review
- Inhibiting the inflammasome: one domain at a time.Immunological reviews · 2015Review
- An updated view on the structure and function of PYRIN domains.Apoptosis : an international journal on programmed cell death · 2015Article
- Activation and assembly of the inflammasomes through conserved protein domain families.Apoptosis : an international journal on programmed cell death · 2015Review
- The CLRX.1/NOD24 (NLRP2P) pseudogene codes a functional negative regulator of NF-κB, pyrin-only protein 4.Genes and immunity · 2014Article
- Pyrin- and CARD-only Proteins as Regulators of NLR Functions.Frontiers in immunology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Activation of transcription factor NF-κB and inflammasome-directed caspase-1 cleavage of IL-1β are key processes in the inflammatory response to pathogen or host-derived signals. Pyrin-only proteins (POPs) are restricted to Old World monkeys, apes, and humans and have previously been shown to impair inflammasome assembly and/or NF-κB p65 transcriptional activity in transfected epithelial cells. However, the biological role of POP2 and the molecular basis for its observed functions are not well understood. In this report we demonstrate that POP2 regulates TNFα and IL-1β responses in human monocytic THP-1 cells and in stable transfectants of mouse J774A.1 macrophages. Deletion analysis of POP2 revealed that the first α-helix (residues 1-19) is necessary and sufficient for both inflammasome and NF-κB inhibitory functions. Further, key acidic residues Glu(6), Asp(8), and Glu(16), believed critical for Pyrin/Pyrin domain interaction, are important for inflammasome inhibition. Moreover, these mutations did not reduce the effect of POP2 upon NF-κB, indicating that the inflammasome and NF-κB inhibitory properties of POP2 can be uncoupled mechanistically. Collectively, these data demonstrate that POP2 acts as a regulator of inflammatory signals and exerts its two known functions through distinct modalities employed by its first α-helix.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.