ArticleJournal of virology2011
Modeling of human cytomegalovirus maternal-fetal transmission in a novel decidual organ culture.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Intrauterine therapy of cytomegalovirus infection with valganciclovir: review of the literature.Medical microbiology and immunology · 2017Pooled it
- Human cytomegalovirus at the maternal-fetal interface: an overview of pathogenesis, defence, and interventions.Clinical science (London, England : 1979) · 2026Review
- Article
- Infections at the maternal-fetal interface: an overview of pathogenesis and defence.Nature reviews. Microbiology · 2022Review
- Intracellular Lipid Accumulation Drives the Differentiation of Decidual Polymorphonuclear Myeloid-Derived Suppressor Cells via Arachidonic Acid Metabolism.Frontiers in immunology · 2022Article
- Virome and metagenomic analysis reveal the distinct distribution of microbiota in human fetal gut during gestation.Frontiers in immunology · 2022Article
- KIR3DL05 and KIR3DS02 Recognition of a Nonclassical MHC Class I Molecule in the Rhesus Macaque Implicated in Pregnancy Success.Frontiers in immunology · 2022Article
- Decidual NK cells kill Zika virus-infected trophoblasts.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Biology and pathology of the uterine microenvironment and its natural killer cells.Cellular & molecular immunology · 2021Review
- Maternal natural killer cells at the intersection between reproduction and mucosal immunity.Mucosal immunology · 2021Review
- Human Nasal and Lung Tissues InfectedJournal of virology · 2021Article
- Trophoblastic extracellular vesicles and viruses: Friends or foes?American journal of reproductive immunology (New York, N.Y. : 1989) · 2021Review
- The Role of Congenital Cytomegalovirus Infection in Adverse Birth Outcomes: A Review of the Potential Mechanisms.Viruses · 2020Review
- Mechanisms and evidence of vertical transmission of infections in pregnancy including SARS-CoV-2s.Prenatal diagnosis · 2020Review
- Human Nasal Turbinate Tissues in Organ Culture as a Model for Human Cytomegalovirus Infection at the Mucosal Entry Site.Journal of virology · 2020Article
- A mutation in POLR3E impairs antiviral immune response and RNA polymerase III.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- Pluripotent Stem Cell-Based Models: A Peephole into Virus Infections during Early Pregnancy.Cells · 2020Review
- Viral-Immune Cell Interactions at the Maternal-Fetal Interface in Human Pregnancy.Frontiers in immunology · 2020Review
- Implications of TORCH Diseases in Retinal Development-Special Focus on Congenital Toxoplasmosis.Frontiers in cellular and infection microbiology · 2020Review
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human cytomegalovirus (HCMV) is the leading cause of congenital infection, associated with severe birth defects and intrauterine growth retardation. The mechanism of HCMV transmission via the maternal-fetal interface is largely unknown, and there are no animal models for HCMV. The initial stages of infection are believed to occur in the maternal decidua. Here we employed a novel decidual organ culture, using both clinically derived and laboratory-derived viral strains, for the ex vivo modeling of HCMV transmission in the maternal-fetal interface. Viral spread in the tissue was demonstrated by the progression of infected-cell foci, with a 1.3- to 2-log increase in HCMV DNA and RNA levels between days 2 and 9 postinfection, the expression of immediate-early and late proteins, the appearance of typical histopathological features of natural infection, and dose-dependent inhibition of infection by ganciclovir and acyclovir. HCMV infected a wide range of cells in the decidua, including invasive cytotrophoblasts, macrophages, and endothelial, decidual, and dendritic cells. Cell-to-cell viral spread was revealed by focal extension of infected-cell clusters, inability to recover infectious extracellular virus, and high relative proportions (88 to 93%) of cell-associated viral DNA. Intriguingly, neutralizing HCMV hyperimmune globulins exhibited inhibitory activity against viral spread in the decidua even when added at 24 h postinfection-providing a mechanistic basis for their clinical use in prenatal prevention. The ex vivo-infected decidual cultures offer unique insight into patterns of viral tropism and spread, defining initial stages of congenital HCMV transmission, and can facilitate evaluation of the effects of new antiviral interventions within the maternal-fetal interface milieu.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.