ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2011
In vivo flow cytometry of circulating clots using negative photothermal and photoacoustic contrasts.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05301348 (In Vivo Detection of Circulating Clots in Patients With Thromboembolism), which is not on this map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
In Vivo Detection of Circulating Clots in Patients With Thromboembolism
Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Deep learning-enabled detection of rare circulating tumor cell clusters in whole blood using label-free, flow cytometry.Lab on a chip · 2024Article
- Deep Learning-Enabled, Detection of Rare Circulating Tumor Cell Clusters in Whole Blood Using Label-free, Flow Cytometry.bioRxiv : the preprint server for biology · 2023Article
- Biomedical Application of Photoacoustics: A Plethora of Opportunities.Micromachines · 2022Review
- Label-free flow cytometry of rare circulating tumor cell clusters in whole blood.Scientific reports · 2022Article
- Towards rainbow portable Cytophone with laser diodes for global disease diagnostics.Scientific reports · 2022Article
- Sound out the impaired perfusion: Photoacoustic imaging in preclinical ischemic stroke.Frontiers in neuroscience · 2022Review
- Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow.Biomedical optics express · 2021Article
- Label-free photoacoustic and ultrasound imaging for murine atherosclerosis characterization.APL bioengineering · 2020Article
- Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches.International journal of molecular sciences · 2020Review
- Combined ultrasound and photoacoustic imaging of blood clot during microbubble-assisted sonothrombolysis.Journal of biomedical optics · 2019Article
- Detection of Apoptotic Circulating Tumor Cells Using in vivo Fluorescence Flow Cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Photoacoustic and fluorescent effects in multilayer plasmon-dye interfaces.Journal of biophotonics · 2019Article
- Technology Advancements in Blood Coagulation Measurements for Point-of-Care Diagnostic Testing.Frontiers in bioengineering and biotechnology · 2019Review
- Noninvasive label-free detection of circulating white and red blood clots in deep vessels with a focused photoacoustic probe.Biomedical optics express · 2018Article
- Dynamic blood flow phantom with negative and positive photoacoustic contrasts.Biomedical optics express · 2018Article
- Tutorial on photoacoustic tomography.Journal of biomedical optics · 2016Review
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- Real-time monitoring of circulating tumor cell release during tumor manipulation using in vivo photoacoustic and fluorescent flow cytometry.Head & neck · 2014Article
- Synergy of photoacoustic and fluorescence flow cytometry of circulating cells with negative and positive contrasts.Journal of biophotonics · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Conventional photothermal (PT) and photoacousic (PA) imaging, spectroscopy, and cytometry are preferentially based on positive PT/PA effects, when signals are above background. Here, we introduce PT/PA technique based on detection of negative signals below background. Among various new applications, we propose label-free in vivo flow cytometry of circulating clots. No method has been developed for the early detection of clots of different compositions as a source of thromboembolism including ischemia at strokes and myocardial infarction. When a low-absorbing, platelet-rich clot passes a laser-irradiated vessel volume, a transient decrease in local absorption results in an ultrasharp negative PA hole in blood background. Using this phenomenon alone or in combination with positive contrasts, we demonstrated identification of white, red, and mixed clots on a mouse model of myocardial infarction and human blood. The concentration and size of clots were measured with threshold down to few clots in the entire circulation with size as low as 20 μm. This multiparameter diagnostic platform using portable personal high-speed flow cytometer with negative dynamic contrast mode has potential to real-time defining risk factors for cardiovascular diseases, and for prognosis and prevention of stroke or use clot count as a marker of therapy efficacy. Possibility for label-free detection of platelets, leukocytes, tumor cells or targeting themby negative PA probes (e.g., nonabsorbing beads or bubbles) is also highlighted.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.