ArticlePflugers Archiv : European journal of physiology2012
Altered expression of tight junction proteins in mammary epithelium after discontinued suckling in mice.
Article in Pflugers Archiv : European journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Colostrogenesis: Role and Mechanism of the Bovine Fc Receptor of the Neonate (FcRn).Journal of mammary gland biology and neoplasia · 2021Review
- Expression of Grainyhead-like 2 in the Process of Ductal Development of Mouse Mammary Gland.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Article
- Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels.International journal of molecular sciences · 2020Article
- Basolateral pressure challenges mammary epithelial cell monolayer integrity, in vitro.Cytotechnology · 2018Article
- Comparative analysis of theophylline and cholera toxin in rat colon reveals an induction of sealing tight junction proteins.Pflugers Archiv : European journal of physiology · 2014Article
- Laurate permeabilizes the paracellular pathway for small molecules in the intestinal epithelial cell model HT-29/B6 via opening the tight junctions by reversible relocation of claudin-5. [Corrected].Pharmaceutical research · 2014Article
- Emerging multifunctional roles of Claudin tight junction proteins in bone.Endocrinology · 2014Review
- The role of tight junctions in mammary gland function.Journal of mammary gland biology and neoplasia · 2014Review
- Tight junction physiology of pleural mesothelium.Frontiers in physiology · 2014Review
- Lactobacillus rhamnosus GG on rotavirus-induced injury of ileal epithelium in gnotobiotic pigs.Journal of pediatric gastroenterology and nutrition · 2013Article
- Claudins and the modulation of tight junction permeability.Physiological reviews · 2013Review
- Lipopolysaccharide disrupts the milk-blood barrier by modulating claudins in mammary alveolar tight junctions.PloS one · 2013Article
- Current trends in salivary gland tight junctions.Tissue barriersReview
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Milk production is modulated by the paracellular barrier function of tight junction (TJ) proteins located in the mammary epithelium. The aim of our study was the molecular analysis of TJs in native lactating murine mammary gland epithelium as this process may strongly challenge epithelial barrier properties and regulation. Mammary gland tissue specimens from lactating control mice and animals after a 20-h interruption of suckling were prepared; histological analyses were performed by light and electron microscopy; and expression of TJ proteins was detected by PCR, Western blotting, immunofluorescent staining, and confocal laser scanning microscopy. Discontinuation of suckling resulted in a substantial accumulation of milk in mammary glands, an increase of alveolar size, and a flattening of epithelial cells without effects on inflammatory indicators. In control tissues, PCR and Western blots showed signals for occludin, and claudin-1, -2, -3, -4, -5, -7, -8, -15, and -16. After a 20-h accumulation of milk, expression of two sealing TJ proteins, claudin-1 and -3, was markedly increased, whereas two TJ proteins involved in cation transport, claudin-2 and -16, were reduced. Real-time PCR validated increased transcripts of claudin-1 and claudin-3. During extension of mammary glands in the process of lactation, claudin-1 and -3 are markedly induced and claudin-2 and -16 are decreased. Volume and composition of milk might be strongly dependent on this counter-regulation of sealing claudins with permeability-mediating claudins, indicating a physiological process of a tightening of TJs against a back-leak of solutes and ions from the alveolar lumen.
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