ArticleJournal of neurophysiology2012
Prestin in HEK cells is an obligate tetramer.
Article in Journal of neurophysiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Stable mammalian expression of His-tagged prestin in Chinese hamster ovary cells.Cytotechnology · 2026Article
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- Single particle cryo-EM structure of the outer hair cell motor protein prestin.Nature communications · 2022Article
- Article
- Article
- Prestin amplifies cardiac motor functions.Cell reports · 2021Article
- A novel theoretical framework reveals more than one voltage-sensing pathway in the lateral membrane of outer hair cells.The Journal of general physiology · 2020Article
- Outer Hair Cells and Electromotility.Cold Spring Harbor perspectives in medicine · 2019Review
- Variable number of TMC1-dependent mechanotransducer channels underlie tonotopic conductance gradients in the cochlea.Nature communications · 2018Article
- Prestin Contributes to Membrane Compartmentalization and Is Required for Normal Innervation of Outer Hair Cells.Frontiers in cellular neuroscience · 2018Article
- The R130S mutation significantly affects the function of prestin, the outer hair cell motor protein.Journal of molecular medicine (Berlin, Germany) · 2016Article
- Assembling the puzzle: Oligomerization of α-pore forming proteins in membranes.Biochimica et biophysica acta · 2016Review
- Susceptibility of outer hair cells to cholesterol chelator 2-hydroxypropyl-β-cyclodextrine is prestin-dependent.Scientific reports · 2016Article
- Glutamate transporter homolog-based model predicts that anion-π interaction is the mechanism for the voltage-dependent response of prestin.The Journal of biological chemistry · 2015Article
- Activity-dependent regulation of prestin expression in mouse outer hair cells.Journal of neurophysiology · 2015Article
- Novel Roles for Chloride Channels, Exchangers, and Regulators in Chronic Inflammatory Airway Diseases.Mediators of inflammation · 2015Review
- Prestin at year 14: progress and prospect.Hearing research · 2014Review
- Molecular architecture and the structural basis for anion interaction in prestin and SLC26 transporters.Nature communications · 2014Article
- Acoustic input and efferent activity regulate the expression of molecules involved in cochlear micromechanics.Frontiers in systems neuroscience · 2014Article
- The conserved tetrameric subunit stoichiometry of Slc26 proteins.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The unusual membrane motor protein prestin is essential for mammalian hearing and for the survival of cochlear outer hair cells. While prestin has been demonstrated to be a homooligomer, by Western blot and FRET analyses, the stoichiometry of self association is unclear. Prestin, coupled to the enhanced green fluorescent protein, was synthesized and membrane targeted in human embryonic kidney cells by plasmid transfection. Fragments of membrane containing immobilized fluorescent molecules were isolated by osmotic lysis. Diffraction-limited fluorescent spots consistent in size with single molecules were observed. Under continuous excitation, the spots bleached to background in sequential and approximately equal-amplitude steps. The average step count to background levels was 2.7. A binomial model of prestin oligomerization indicated that prestin was most likely a tetramer, and that a fraction of the green fluorescent protein molecules was dark. As a positive control, the same procedure was applied to cells transfected with plasmids coding for the human cyclic nucleotide-gated ion channel A3 subunit (again coupled to the enhanced green fluorescent protein), which is an obligate tetramer. The average step count for this molecule was also 2.7. This result implies that in cell membranes prestin oligomerizes to a tetramer.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.