Evidence map›Paper›PMID 21975125›Full record

ArticleVirology journal2011

Epstein - Barr virus Latent Membrane Protein 1 suppresses reporter activity through modulation of promyelocytic leukemia protein-nuclear bodies.

Mark D Sides, Gregory J Block, Reid W Chadwick, Bin Shan, Erik K Flemington, Joseph A Lasky

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 87% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Mark D SidesDepartment of Medicine, Section of Pulmonary Disease and Critical Care, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA 70112, USA.
Gregory J Block
Reid W Chadwick
Bin Shan
Erik K Flemington
Joseph A Lasky
Tulane University · USUniversity of Washington · US

Funding

Role of the cellular microRNA, miR-155, in EBV type III latency signalingR01CA138268 · NCI · TULANE UNIVERSITY OF LOUISIANA · PI FLEMINGTON, ERIK K · 2009 to 2013
$1.5M
NCI NIH HHS R01 CA138268
6 · The paper itself

Abstract

The Epstein-Barr virus (EBV) encoded Latent Membrane Protein 1 (LMP1) has been shown to increase the expression of promyelocytic leukemia protein (PML) and the immunofluorescent intensity of promyelocytic leukemia nuclear bodies (PML NBs). PML NBs have been implicated in the modulation of transcription and the association of reporter plasmids with PML NBs has been implicated in repression of reporter activity. Additionally, repression of various reporters in the presence of LMP1 has been noted. This study demonstrates that LMP1 suppresses expression of reporter activity in a dose responsive manner and corresponds with the LMP1 induced increase in PML NB intensity. Disruption of PML NBs with arsenic trioxide or a PML siRNA restores reporter activity. These data offer an explanation for previously conflicting data on LMP1 signaling and calls attention to the possibility of false-positives and false-negatives when using reporter assays as a research tool in cells expressing LMP1.

Indexed as

ArsenicalsArsenic Trioxidebeta-GalactosidaseCell LineDose-Response Relationship, DrugEpstein-Barr Virus InfectionsGene Expression Regulation, LeukemicGene Expression Regulation, ViralGene SilencingGenes, ReporterHerpesvirus 4, HumanHumansIntranuclear Inclusion BodiesLeukemia, Promyelocytic, AcuteLuciferasesNuclear ProteinsArsenicalsArsenic Trioxidebeta-GalactosidaseEBV-associated membrane antigen, Epstein-Barr virusLuciferasesNuclear ProteinsOxidesPML protein, humanPromyelocytic Leukemia ProteinRNA, Small InterferingTranscription FactorsTumor Suppressor ProteinsViral Matrix Proteins

Identifiers

PMID21975125
PMCPMC3204298
OpenAlexW2147151327

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.