ArticlePlant physiology2011
Posttranslational regulation of the iron deficiency basic helix-loop-helix transcription factor FIT is affected by iron and nitric oxide.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
67 citing papers in PubMed, 149 citations in OpenAlex.
- CITF1 interacts with FIT and regulates copper-iron crosstalk in Arabidopsis.The Plant cell · 2026Article
- Nitric oxide donor sodium nitroprusside enhances iron deficiency tolerance in M7 apple plants through coordinated regulation of Fe acquisition genes and physiological responses.Scientific reports · 2026Article
- The Volatile Pentadecane From Bacillus Alleviates Plant Iron Deficiency Through Activating the Reduction-Based Fe Uptake System.Plant biotechnology journal · 2026Article
- Age-dependent differential iron deficiency responses of rosette leaves during reproductive stages in Arabidopsis thaliana.Journal of experimental botany · 2025Article
- The transcription factor MYB30 promotes iron homeostasis by maintaining the stability of the FIT transcription factor.The Plant cell · 2025Article
- Copper connections: coordinating transport, sensing and systemic signalling in plants.Quantitative plant biology · 2025Review
- Micronutrient deficiency-induced oxidative stress in plants.Plant cell reports · 2024Review
- Signaling function of NHPlanta · 2024Article
- FER-like iron deficiency-induced transcription factor (FIT) accumulates in nuclear condensates.The Journal of cell biology · 2024Article
- Calcium-dependent protein kinases CPK21 and CPK23 phosphorylate and activate the iron-regulated transporter IRT1 to regulate iron deficiency in Arabidopsis.Science China. Life sciences · 2023Article
- Cellular clarity: a logistic regression approach to identify root epidermal regulators of iron deficiency response.BMC genomics · 2023Article
- NO Is Not the Same as GSNO in the Regulation of Fe Deficiency Responses by Dicot Plants.International journal of molecular sciences · 2023Review
- Nitric Oxide and Globin Glb1 RegulateAntioxidants (Basel, Switzerland) · 2023Article
- Iron Availability and Homeostasis in Plants: A Review of Responses, Adaptive Mechanisms, and Signaling.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Iron uptake, signaling, and sensing in plants.Plant communications · 2022Review
- Alleviation of iron deficiency in pear by ammonium nitrate and nitric oxide.BMC plant biology · 2022Article
- Nitric oxide signalling in roots is required for MYB72-dependent systemic resistance induced by Trichoderma volatile compounds in Arabidopsis.Journal of experimental botany · 2022Article
- A shoot derived long distance iron signal may act upstream of the IMA peptides in the regulation of Fe deficiency responses inFrontiers in plant science · 2022Article
- Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach.Frontiers in nutrition · 2022Article
- IRONMAN tunes responses to iron deficiency in concert with environmental pH.Plant physiology · 2021Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding iron (Fe) sensing and regulation is important for targeting key genes for important nutritional traits like Fe content. The basic helix-loop-helix transcription factor FIT (for FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) controls Fe acquisition genes in dicot roots. Posttranscriptional regulation of transcription factors allows rapid adaptation to cellular changes and was also described for FIT. However, the mechanisms behind this regulation of FIT were for a long time not known. Here, we studied the posttranscriptional control mechanisms of FIT in Arabidopsis (Arabidopsis thaliana) and identified nitric oxide as a stabilizing stimulus for FIT protein abundance. Using cycloheximide, we confirmed that the level of FIT protein was regulated by way of protein turnover in wild-type and hemagglutinin-FIT plants. Upon cycloheximide treatment, FIT activity was hardly compromised, since Fe deficiency genes like IRON-REGULATED TRANSPORTER1 and FERRIC REDUCTASE OXIDASE2 were still inducible by Fe deficiency. A small pool of "active" FIT was sufficient for the induction of Fe deficiency downstream responses. Nitric oxide inhibitors caused a decrease of FIT protein abundance and, in the wild type, also a decrease in FIT activity. This decrease of FIT protein levels was reversed by the proteasomal inhibitor MG132, suggesting that in the presence of nitric oxide FIT protein was less likely to be a target of proteasomal degradation. Independent of FIT transcription, FIT protein stability and FIT protein activity, therefore, were targets of control mechanisms in response to Fe and nitric oxide. We summarize our results in a model that explains the different steps of FIT regulation integrating the plant signals that control FIT.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.