ArticlePlanta2012
An ER-targeted calcium-binding peptide confers salt and drought tolerance mediated by CIPK6 in Arabidopsis.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- LjCIPK1: a CBL-interacting protein kinase fromFrontiers in plant science · 2026Article
- Characterization of McCIPK6, a mitochondrial protein, positively regulating cold stress tolerance in bitter gourd (Frontiers in plant science · 2026Article
- Role of Abscisic Acid, Reactive Oxygen Species, and CaPlants (Basel, Switzerland) · 2024Review
- Comparative analysis of wild-type and chloroplast MCU-deficient plants reveals multiple consequences of chloroplast calcium handling under drought stress.Frontiers in plant science · 2023Article
- Combining Physio-Biochemical Characterization and Transcriptome Analysis Reveal the Responses to Varying Degrees of Drought Stress inInternational journal of molecular sciences · 2022Article
- Identification, expression, and association analysis of calcineurin B-like protein-interacting protein kinase genes in peanut.Frontiers in genetics · 2022Article
- HalophyteFrontiers in plant science · 2022Article
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- Genome-Wide Identification, Characterization and Expression Analysis of theInternational journal of molecular sciences · 2021Article
- New insights into the evolution and functional divergence of the CIPK gene family in Saccharum.BMC genomics · 2020Article
- The CBL-CIPK Pathway in Plant Response to Stress Signals.International journal of molecular sciences · 2020Review
- Linking Duplication of a Calcium Sensor to Salt Tolerance inPlant physiology · 2019Article
- An apple sucrose transporter MdSUT2.2 is a phosphorylation target for protein kinase MdCIPK22 in response to drought.Plant biotechnology journal · 2019Article
- Functional analysis of MeCIPK23 and MeCBL1/9 in cassava defense response against Xanthomonas axonopodis pv. manihotis.Plant cell reports · 2018Article
- Integrated regulation triggered by a cryophyte ω-3 desaturase gene confers multiple-stress tolerance in tobacco.Journal of experimental botany · 2018Article
- CBL-interacting protein kinase 6 negatively regulates immune response to Pseudomonas syringae in Arabidopsis.Journal of experimental botany · 2017Article
- A Universal Stress Protein Involved in Oxidative Stress Is a Phosphorylation Target for Protein Kinase CIPK6.Plant physiology · 2017Article
- Expression of chickpea CIPK25 enhances root growth and tolerance to dehydration and salt stress in transgenic tobacco.Frontiers in plant science · 2015Article
- Genome-wide gene phylogeny of CIPK family in cassava and expression analysis of partial drought-induced genes.Frontiers in plant science · 2015Article
- Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Different plant organelles have high internal stores of Ca(2+) compared to the cytoplasm and could play independent roles in stress responses or signal transduction. We used a GFP fusion with the C-domain of calreticulin, which shows low-affinity, high capacity Ca(2+) binding in the ER, as a calcium-binding peptide (CBP) to specifically increase stores in the ER and nucleus. Despite the presence of a signal sequence and KDEL retention sequence, our work and previous studies (Brandizzi et al. Plant Journal 34:269-281, 2003) demonstrated both ER and nuclear localization of GFP-CBP. Under normal conditions, GFP-CBP-expressing lines had ~25% more total Ca(2+) and higher levels of chlorophyll and seed yield than wild type and GFP controls. CBP-expressing plants also had better survival under intermittent drought or high salt treatments and increased root growth. One member of the CIPK (calcineurin B-like interacting protein kinase) gene family, CIPK6, was up-regulated in CBP-expressing plants, even under non-stress conditions. A null mutation in cipk6 abolished the increased stress tolerance of CBP-transgenic plants, as well as the CBP-mediated induction of two stress-associated genes, DREB1A and RD29A, under non-stress conditions. Although this suggested that it was the induction of CIPK6, rather than localized changes in Ca(2+), that resulted in increased survival under adverse conditions, CIPK6 induction still required Ca(2+). This work demonstrates that ER (or nuclear) Ca(2+) can directly participate in signal transduction to alter gene expression. The discovery of a method for increasing Ca(2+) levels without deleterious effects on plant growth may have practical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.