ArticleMycopathologia2012
Expression and characterization of Cryptococcus neoformans recombinant App1.
Article in Mycopathologia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Advances in cryptococcal infections-associated immunopathology and potential therapeutic strategies.Frontiers in pharmacology · 2026Review
- Identification of a protective antigen reveals the trade-off between iron acquisition and antigen exposure in a global fungal pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Dangerous Liaisons: Interactions ofPathogens (Basel, Switzerland) · 2020Review
- Cell Wall-Associated Virulence Factors Contribute to Increased Resilience of OldFrontiers in microbiology · 2019Article
- Cryptococcus and cryptococcosis in the twenty-first century.Mycopathologia · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We characterized Cryptococcus neoformans recombinant antiphagocytic protein 1 (rApp1) by SDS-PAGE, gel filtration chromatography, circular dichroism, and fluorescence spectroscopy. rApp1 produced by C. neoformans var. grubii contains an odd number of cysteines; therefore, it has the ability to form intermolecular disulfide bridges which can lead to the formation of amyloid fibrils in the absence of β-mercaptoethanol or DTT in vitro. Alternate approaches to over-expression of rApp1 in the Lepidopteran High Five(™) Insect cell line using pIZ/V5-His and in lentivirus were explored and are described. Finally, localization of App1 in vivo was examined in the presence and absence of the capsule.
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