ArticleResuscitation2012
Microglial depletion using intrahippocampal injection of liposome-encapsulated clodronate in prolonged hypothermic cardiac arrest in rats.
Article in Resuscitation, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Peripheral monocytes and neutrophils promote photoreceptor cell death in an experimental retinal detachment model.Cell death & disease · 2023Article
- Flufenamic acid improves survival and neurologic outcome after successful cardiopulmonary resuscitation in mice.Journal of neuroinflammation · 2022Article
- A method for the selective depletion of microglia in the dorsal hippocampus in the juvenile rat brain.Journal of neuroscience methods · 2022Article
- Article
- The Effect of a Modified Constant Flow Insufflation of Oxygen during Cardiopulmonary Resuscitation in a Rat Model of Respiratory Cardiac Arrest on Arterial Oxygenation, Alveolar Barotrauma, and Brain Tissue Injury.Emergency medicine international · 2020Article
- Microglia in the developing retina.Neural development · 2019Review
- Depletion of microglia immediately following traumatic brain injury in the pediatric rat: Implications for cellular and behavioral pathology.Experimental neurology · 2019Article
- Rat Cerebrospinal Fluid Treatment Method through Cisterna Cerebellomedullaris Injection.Neuroscience bulletin · 2018Article
- Lappaconitine, a C18-diterpenoid alkaloid, exhibits antihypersensitivity in chronic pain through stimulation of spinal dynorphin A expression.Psychopharmacology · 2018Article
- (+)-Naltrexone is neuroprotective and promotes alternative activation in the mouse hippocampus after cardiac arrest/cardiopulmonary resuscitation.Brain, behavior, and immunity · 2015Article
- Effect of Tempol on Cerebral Resuscitation Caused by Asphyxia-Induced Cardiac Arrest.Acta Cardiologica Sinica · 2015Article
- Extracorporeal versus conventional cardiopulmonary resuscitation after ventricular fibrillation cardiac arrest in rats: a feasibility trial.Critical care medicine · 2013Article
- Targeting glioblastoma with NK cells and mAb against NG2/CSPG4 prolongs animal survival.Oncotarget · 2013Article
- Microglia regulate the number of neural precursor cells in the developing cerebral cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- The brain's best friend: microglial neurotoxicity revisited.Frontiers in cellular neuroscience · 2013Article
- Characterization of status epilepticus induced by two organophosphates in rats.Epilepsy research · 2012Article
- State-of-the-art methods for the treatment of severe hemorrhagic trauma: selective aortic arch perfusion and emergency preservation and resuscitation-what is next?Acute medicine & surgeryReview
- Peripheral and Central Administration of Soluble Glycoprotein 130 Improves Cognitive Outcomes Following Controlled Cortical Impact in Male Mice.Neurotrauma reportsArticle
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
Trauma patients who suffer cardiac arrest (CA) from exsanguination rarely survive. Emergency preservation and resuscitation using hypothermia was developed to buy time for resuscitative surgery and delayed resuscitation with cardiopulmonary bypass (CPB), but intact survival is limited by neuronal death associated with microglial proliferation and activation. Pharmacological modulation of microglia may improve outcome following CA. Systemic injection of liposome-encapsulated clodronate (LEC) depletes macrophages. To test the hypothesis that intrahippocampal injection of LEC would attenuate local microglial proliferation after CA in rats, we administered LEC or PBS into the right or left hippocampus, respectively. After rapid exsanguination and 6min no-flow, hypothermia was induced by ice-cold (IC) or room-temperature (RT) flush. Total duration of CA was 20min. Pre-treatment (IC, RTpre) and post-treatment (RTpost) groups were studied, along with shams (cannulation only) and CPB controls. On day 7, shams and CPB groups showed neither neuronal death nor microglial activation. In contrast, the number of microglia in hippocampus in each individual group (IC, RTpre, RTpost) was decreased with LEC vs. PBS by ∼34-46% (P<0.05). Microglial proliferation was attenuated in the IC vs. RT groups (P<0.05). Neuronal death did not differ between hemispheres or IC vs. RT groups. Thus, intrahippocampal injection of LEC attenuated microglial proliferation by ∼40%, but did not alter neuronal death. This suggests that microglia may not play a pivotal role in mediating neuronal death in prolonged hypothermic CA. This novel strategy provides us with a tool to study the specific effects of microglia in hypothermic CA.
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