ArticleProceedings of the National Academy of Sciences of the United States of America2011
Redox cycling and kinetic analysis of single molecules of solution-phase nitrite reductase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The Long-Range High-pH Proton Channel Facilitates the Nitrite Reduction in Copper-Containing Nitrite Reductase.ACS omega · 2025Article
- Evidence of a Three-State Mechanism in DNA Hairpin Folding.Journal of the American Chemical Society · 2025Article
- Revving up a Designed Copper Nitrite Reductase Using Non-Coded Active Site Ligands.ACS catalysis · 2024Article
- Ratiometric Sensing of Redox Environments Inside Individual Carboxysomes Trapped in Solution.The journal of physical chemistry letters · 2022Article
- Single electron transfer events and dynamical heterogeneity in the small protein azurin fromChemical science · 2019Article
- Engineering and Modeling the Electrophoretic Trapping of a Single Protein Inside a Nanopore.ACS nano · 2019Article
- Interferometric Scattering Enables Fluorescence-Free Electrokinetic Trapping of Single Nanoparticles in Free Solution.Nano letters · 2019Article
- A QM/MM Study of Nitrite Binding Modes in a Three-Domain Heme-Cu Nitrite Reductase.Molecules (Basel, Switzerland) · 2018Article
- Article
- Single-molecule spectroscopy and imaging over the decades.Faraday discussions · 2015Review
- Shedding light on protein folding, structural and functional dynamics by single molecule studies.Molecules (Basel, Switzerland) · 2014Review
- Tracking electrons in biological macromolecules: from ensemble to single molecule.Molecules (Basel, Switzerland) · 2014Review
- Copper active sites in biology.Chemical reviews · 2014Review
- Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.Biochemistry · 2013Review
- Characterization of a nitrite reductase involved in nitrifier denitrification.The Journal of biological chemistry · 2013Article
- Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.Analytical chemistry · 2013Review
- Probing single biomolecules in solution using the anti-Brownian electrokinetic (ABEL) trap.Accounts of chemical research · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-molecule measurements are a valuable tool for revealing details of enzyme mechanisms by enabling observation of unsynchronized behavior. However, this approach often requires immobilizing the enzyme on a substrate, a process which may alter enzyme behavior. We apply a microfluidic trapping device to allow, for the first time, prolonged solution-phase measurement of single enzymes in solution. Individual redox events are observed for single molecules of a blue nitrite reductase and are used to extract the microscopic kinetic parameters of the proposed catalytic cycle. Changes in parameters as a function of substrate concentration are consistent with a random sequential substrate binding mechanism.
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