ArticleThe Journal of biological chemistry2011
Direct interaction of mitochondrial targeting presequences with purified components of the TIM23 protein complex.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- Two domains of Tim50 coordinate translocation of proteins across the two mitochondrial membranes.Life science alliance · 2023Article
- Interactions of amyloidogenic proteins with mitochondrial protein import machinery in aging-related neurodegenerative diseases.Frontiers in physiology · 2023Review
- Mitochondrial protein translocation machinery: From TOM structural biogenesis to functional regulation.The Journal of biological chemistry · 2022Review
- Mapping protein interactions in the active TOM-TIM23 supercomplex.Nature communications · 2021Article
- Interplay between Mitochondrial Protein Import and Respiratory Complexes Assembly in Neuronal Health and Degeneration.Life (Basel, Switzerland) · 2021Review
- Coordinated Translocation of Presequence-Containing Precursor Proteins Across Two Mitochondrial Membranes: Knowns and Unknowns of How TOM and TIM23 Complexes Cooperate With Each Other.Frontiers in physiology · 2021Review
- Mgr2 regulates mitochondrial preprotein import by associating with channel-forming Tim23 subunit.Molecular biology of the cell · 2020Article
- Inner Mitochondrial Translocase Tim50 Is Central in Adrenal and Testicular Steroid Synthesis.Molecular and cellular biology · 2019Article
- Hsp70 at the membrane: driving protein translocation.BMC biology · 2018Review
- Lysine desuccinylase SIRT5 binds to cardiolipin and regulates the electron transport chain.The Journal of biological chemistry · 2017Article
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- How lipids modulate mitochondrial protein import.Journal of bioenergetics and biomembranes · 2016Review
- Loss of TIM50 suppresses proliferation and induces apoptosis in breast cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Role of membrane contact sites in protein import into mitochondria.Protein science : a publication of the Protein Society · 2015Review
- Presequence recognition by the tom40 channel contributes to precursor translocation into the mitochondrial matrix.Molecular and cellular biology · 2014Article
- Molecular insights revealing interaction of Tim23 and channel subunits of presequence translocase.Molecular and cellular biology · 2013Article
- Structural changes in the mitochondrial Tim23 channel are coupled to the proton-motive force.Nature structural & molecular biology · 2013Article
- Signal recognition initiates reorganization of the presequence translocase during protein import.The EMBO journal · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precursor proteins that are imported from the cytosol into the matrix of mitochondria carry positively charged amphipathic presequences and cross the inner membrane with the help of vital components of the TIM23 complex. It is currently unclear which subunits of the TIM23 complex recognize and directly bind to presequences. Here we analyzed the binding of presequence peptides to purified components of the TIM23 complex. The interaction of three different presequences with purified soluble domains of yeast Tim50 (Tim50IMS), Tim23 (Tim23IMS), and full-length Tim44 was examined. Using chemical cross-linking and surface plasmon resonance we demonstrate, for the first time, the ability of purified Tim50IMS and Tim44 to interact directly with the yeast Hsp60 presequence. We also analyzed their interaction with presequences derived from precursors of yeast mitochondrial 70-kDa heat shock protein (mHsp70) and of bovine cytochrome P450SCC. Moreover, we characterized the nature of the interactions and determined their KDs. On the basis of our results, we suggest a mechanism of translocation where stronger interactions of the presequences on the trans side of the channel support the import of precursor proteins through TIM23 into the matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.