ArticleInvestigative ophthalmology & visual science2011
Neurotransmitter influence on human meibomian gland epithelial cells.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 61 citations in OpenAlex.
- Review
- Ocular and Neurological Sequelae in Long COVID: Dry Eye, Asthenopia, Sleep Disorders, Asthenia, and Restless Legs Syndrome-A Case Report with Literature Review.Life (Basel, Switzerland) · 2025Article
- Identification of Meibomian gland stem cell populations and mechanisms of aging.Nature communications · 2025Article
- Identification of Meibomian gland stem cell populations and mechanisms of aging.bioRxiv : the preprint server for biology · 2024Article
- Evaluation of the Clinical Characteristics of Dry Eye Secondary to Different Types of Liver Diseases.Ophthalmology and therapy · 2023Article
- Recent advances in age-related meibomian gland dysfunction (ARMGD).The ocular surface · 2023Review
- Ocular surface disease: a known yet overlooked side effect of topical glaucoma therapy.Frontiers in toxicology · 2023Review
- Ocular surface alterations and changes of meibomian glands with meibography in type 1 diabetic children.International ophthalmology · 2022Article
- Mechanistic investigations of diabetic ocular surface diseases.Frontiers in endocrinology · 2022Review
- Role of Choline in Ocular Diseases.International journal of molecular sciences · 2021Review
- Cutaneous and ocular rosacea: Common and specific physiopathogenic mechanisms and study models.Molecular vision · 2021Review
- Article
- [Intense pulsed light (IPL) as a therapeutic option for Meibomian gland dysfunction].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2019Review
- Meibocyte differentiation and renewal: Insights into novel mechanisms of meibomian gland dysfunction (MGD).Experimental eye research · 2017Review
- Meibomian glands, meibum, and meibogenesis.Experimental eye research · 2017Review
- TFOS DEWS II pain and sensation report.The ocular surface · 2017Review
- Article
- Review of autoantigens in Sjögren's syndrome: an update.Journal of inflammation research · 2017Review
- PPARγ Regulates Mouse Meibocyte Differentiation and Lipid Synthesis.The ocular surface · 2016Article
- Serum-induced differentiation of human meibomian gland epithelial cells.Investigative ophthalmology & visual science · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeA striking characteristic of the human meibomian gland is its rich sensory, sympathetic, and parasympathetic innervation, yet the functional relevance of these nerve fibers remains unknown. Acting on the hypothesis that neurotransmitters are released in the vicinity of the gland, act on glandular receptors, and influence the production, secretion, and/or delivery of meibomian gland secretions to the ocular surface, the goal in this study was to begin to determine whether neurotransmitters influence the meibomian gland.
methodsImmortalized human meibomian gland epithelial (SLHMG) cells were examined for the presence of vasoactive intestinal peptide (VIP) and muscarinic acetylcholine (mACh) receptor transcripts and proteins. Cells were also exposed to VIP, carbachol, forskolin, and/or 3-isobutyl-1-methylxanthine (IBMX) to determine whether these agents, alone or in combination, modulate the adenylyl cyclase pathway, the accumulation of intracellular free calcium ([Ca2+]i), or cell proliferation.
resultsResults demonstrate that SLHMG cells transcribe and translate VIP and mACh receptors; VIP, with either IBMX or forskolin, activates the adenylyl cyclase pathway, and the effect of VIP and forskolin together is synergistic; both VIP and carbachol increase intracellular [Ca2+] in SLHMG cells; and VIP with forskolin stimulates SLHMG cell proliferation.
conclusionsThis study shows that parasympathetic neurotransmitters and their agonists influence the function of human meibomian gland epithelial cells. It remains to be determined whether this action alters the production, secretion, and/or delivery of meibum to the ocular surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.