ArticleJournal of molecular neuroscience : MN2012
Optimized quantities of GDNF overexpressed by engineered astrocytes are critical for protection of neuroblastoma cells against 6-OHDA toxicity.
Article in Journal of molecular neuroscience : MN, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Tuning Secretomes for Regenerative Medicine.Biology · 2026Review
- Glial cells in Parkinson´s disease: protective or deleterious?Cellular and molecular life sciences : CMLS · 2020Review
- Growth Factors and Neuroglobin in Astrocyte Protection Against Neurodegeneration and Oxidative Stress.Molecular neurobiology · 2019Review
- PDGF-BB Preserves Mitochondrial Morphology, Attenuates ROS Production, and Upregulates Neuroglobin in an Astrocytic Model Under Rotenone Insult.Molecular neurobiology · 2018Article
- Novel Approaches in Astrocyte Protection: from Experimental Methods to Computational Approaches.Journal of molecular neuroscience : MN · 2016Review
- PDGF-BB protects mitochondria from rotenone in T98G cells.Neurotoxicity research · 2015Article
- Glutathione transferase-M2-2 secreted from glioblastoma cell protects SH-SY5Y cells from aminochrome neurotoxicity.Neurotoxicity research · 2015Article
- Lenti-GDNF gene therapy protects against Alzheimer's disease-like neuropathology in 3xTg-AD mice and MC65 cells.CNS neuroscience & therapeutics · 2014Article
- Glutathione transferase mu 2 protects glioblastoma cells against aminochrome toxicity by preventing autophagy and lysosome dysfunction.Autophagy · 2014Article
- The proform of glia cell line-derived neurotrophic factor: a potentially biologically active protein.Molecular neurobiology · 2014Article
- Astrocytic modulation of blood brain barrier: perspectives on Parkinson's disease.Frontiers in cellular neuroscience · 2014Review
- N-cadherin is a novel ERα anchor that protects against 6-OHDA damage to dopaminergic cells.Cellular and molecular neurobiology · 2014Article
- Shikonin protects dopaminergic cell line PC12 against 6-hydroxydopamine-mediated neurotoxicity via both glutathione-dependent and independent pathways and by inhibiting apoptosis.Neurochemical research · 2013Article
- Evaluation of the neurotoxic/neuroprotective role of organoselenides using differentiated human neuroblastoma SH-SY5Y cell line challenged with 6-hydroxydopamine.Neurotoxicity research · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optimized levels of glial cell line-derived neurotrophic factor (GDNF) are critical for protection of dopaminergic neurons against parkinsonian cell death. Recombinant lentiviruses harboring GDNF coding sequence were constructed and used to infect astrocytoma cell line 1321N1. The infected astrocytes overexpressed GDNF mRNA and secreted an average of 2.2 ng/mL recombinant protein as tested in both 2 and 16 weeks post-infection. Serial dilutions of GDNF-enriched conditioned medium from infected astrocytes added to growing neuroblastoma cell line SK-N-MC resulted in commensurate resistance against 6-OHDA toxicity. SK-N-MC cell survival rate rose from 51% in control group to 84% in the cells grown with astro-CM containing 453 pg secreted GDNF, an increase that was highly significant (P < 0.0001). However, larger volumes of the GDNF-enriched conditioned medium failed to improve cell survival and addition of volumes that contained 1,600 pg or more GDNF further reduced survival rate to below 70%. Changes in cell survival paralleled to changes in the percent of apoptotic cell morphologies. These data demonstrate the feasibility of using astrocytes as minipumps to stably oversecrete neurotrophic factors and further indicate that GDNF can be applied to neuroprotection studies in PD pending the optimization of its concentrations.
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