ArticleJournal of visualized experiments : JoVE2011
Detection of histone modifications in plant leaves.
Article in Journal of visualized experiments : JoVE, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Integrated Benefits to Agriculture withMicroorganisms · 2024Review
- Pathogen-Associated Molecular Pattern-Triggered Immunity Involves Proteolytic Degradation of Core Nonsense-Mediated mRNA Decay Factors During the Early Defense Response.The Plant cell · 2020Article
- Benefits to Plant Health and Productivity From Enhancing Plant Microbial Symbionts.Frontiers in plant science · 2020Review
- Pseudo-chromosome-length genome assembly of a double haploid "Bartlett" pear (Pyrus communis L.).GigaScience · 2019Article
- H3K4/K9 acetylation and Lr28-mediated expression of six leaf rust responsive genes in wheat (Triticum aestivum).Molecular genetics and genomics : MGG · 2019Article
- Sulforaphane Modifies Histone H3, Unpacks Chromatin, and Primes Defense.Plant physiology · 2018Article
- The reduction in maize leaf growth under mild drought affects the transition between cell division and cell expansion and cannot be restored by elevated gibberellic acid levels.Plant biotechnology journal · 2018Article
- Determination of histone epigenetic marks in Arabidopsis and tomato genes in the early response to Botrytis cinerea.Plant cell reports · 2018Article
- BABA-Primed Histone Modifications in Potato for Intergenerational Resistance toFrontiers in plant science · 2018Article
- Regulatory cis-elements are located in accessible promoter regions of the CAT2 promoter and affect activating histone modifications in Arabidopsis thaliana.Plant molecular biology · 2017Article
- The RNA Polymerase-Associated Factor 1 Complex Is Required for Plant Touch Responses.Journal of experimental botany · 2017Article
- The 5'UTR Intron of Arabidopsis GGT1 Aminotransferase Enhances Promoter Activity by Recruiting RNA Polymerase II.Plant physiology · 2016Article
- HISTONE DEACETYLASE6 Controls Gene Expression Patterning and DNA Methylation-Independent Euchromatic Silencing.Plant physiology · 2015Article
- One-step purification of twin-strep-tagged proteins and their complexes on strep-tactin resin cross-linked with bis(sulfosuccinimidyl) suberate (BS3).Journal of visualized experiments : JoVE · 2014Article
- Recent advances in maize nuclear proteomic studies reveal histone modifications.Frontiers in plant science · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin structure is important for the regulation of gene expression in eukaryotes. In this process, chromatin remodeling, DNA methylation, and covalent modifications on the amino-terminal tails of histones H3 and H4 play essential roles(1-2). H3 and H4 histone modifications include methylation of lysine and arginine, acetylation of lysine, and phosphorylation of serine residues(1-2). These modifications are associated either with gene activation, repression, or a primed state of gene that supports more rapid and robust activation of expression after perception of appropriate signals (microbe-associated molecular patterns, light, hormones, etc.)(3-7). Here, we present a method for the reliable and sensitive detection of specific chromatin modifications on selected plant genes. The technique is based on the crosslinking of (modified) histones and DNA with formaldehyde(8,9), extraction and sonication of chromatin, chromatin immunoprecipitation (ChIP) with modification-specific antibodies(9,10), de-crosslinking of histone-DNA complexes, and gene-specific real-time quantitative PCR. The approach has proven useful for detecting specific histone modifications associated with C(4;) photosynthesis in maize(5,11) and systemic immunity in Arabidopsis(3).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.