ReviewNucleic acids research2012
Luminescent detection of DNA-binding proteins.
Review in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 97 citations in OpenAlex.
- A fluorescent probe under the microscope showing dual recognition of B-DNA and G-quadruplex DNA.Communications chemistry · 2026Article
- Unraveling Bacterial Single-Stranded Sequence Specificities: Insights from Molecular Dynamics and MMPBSA Analysis of Oligonucleotide Probes.Molecular biotechnology · 2024Review
- Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexes.BMC chemistry · 2020Article
- DNB-based on-chip motif finding: A high-throughput method to profile different types of protein-DNA interactions.Science advances · 2020Article
- A DNA-Based Assay for Digoxin Detection.Biosensors · 2018Article
- Review
- Application of iridium(III) complex in label-free and non-enzymatic electrochemical detection of hydrogen peroxide based on a novel "on-off-on" switch platform.Scientific reports · 2016Article
- Inhibition of the p53/hDM2 protein-protein interaction by cyclometallated iridium(III) compounds.Oncotarget · 2016Article
- iPPBS-Opt: A Sequence-Based Ensemble Classifier for Identifying Protein-Protein Binding Sites by Optimizing Imbalanced Training Datasets.Molecules (Basel, Switzerland) · 2016Article
- Materiomics for Oral Disease Diagnostics and Personal Health Monitoring: Designer Biomaterials for the Next Generation Biomarkers.Omics : a journal of integrative biology · 2016Article
- An Ir(III) complex chemosensor for the detection of thiols.Science and technology of advanced materials · 2016Article
- Prediction of Protein Structural Class Based on Gapped-Dipeptides and a Recursive Feature Selection Approach.International journal of molecular sciences · 2015Article
- A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-HgScience and technology of advanced materials · 2015Article
- A ruthenium(II) complex supported by trithiacyclononane and aromatic diimine ligand as luminescent switch-on probe for biomolecule detection and protein staining.Scientific reports · 2014Article
- Synthesis, labeling and bioanalytical applications of a tris(2,2'-bipyridyl)ruthenium(II)-based electrochemiluminescence probe.Nature protocols · 2014Article
- A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factors play a central role in cell development, differentiation and growth in biological systems due to their ability to regulate gene expression by binding to specific DNA sequences within the nucleus. The dysregulation of transcription factor signaling has been implicated in the pathogenesis of a number of cancers, developmental disorders, inflammation and autoimmunity. There is thus a high demand for convenient high-throughput methodologies able to detect sequence-specific DNA-binding proteins and monitor their DNA-binding activities. Traditional approaches for protein detection include gel mobility shift assays, DNA footprinting and enzyme-linked immunosorbent assays (ELISAs) which tend to be tedious, time-consuming, and may necessitate the use of radiographic labeling. By contrast, luminescence technologies offer the potential for rapid, sensitive and low-cost detection that are amenable to high-throughput and real-time analysis. The discoveries of molecular beacons and aptamers have spear-headed the development of new luminescent methodologies for the detection of proteins over the last decade. We survey here recent advances in the development of luminescent detection methods for DNA-binding proteins, including those based on molecular beacons, aptamer beacons, label-free techniques and exonuclease protection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.