ArticleVirology journal2011
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Hepatitis C virus genotyping based on Core and NS5B regions in Cameroonian patients.Virology journal · 2019Article
- Infection with multiple hepatitis C virus genotypes detected using commercial tests should be confirmed using next generation sequencing.Scientific reports · 2019Article
- Hepatitis C Genotype Prevalence in Monastir Region, Tunisia: Correlation between 5' Untranslated Region (5'UTR), Non-structural 5B (NS5B), and Core Sequences in HCV Subtyping.Current microbiology · 2016Article
- Detection of hepatitis C virus in patients with terminal renal disease undergoing dialysis in southern Brazil: prevalence, risk factors, genotypes, and viral load dynamics in hemodialysis patients.Virology journal · 2015Article
- Oxidative stress response in patients infected by diverse hepatitis C virus genotypes.Hepatitis monthly · 2015Article
- Molecular Assay and Genotyping of Hepatitis C Virus among Infected Egyptian and Saudi Arabian Patients.Virology : research and treatment · 2015Article
- Serological assay and genotyping of hepatitis C virus in infected patients in zanjan province.Hepatitis monthly · 2014Article
- Sequencing assays for failed genotyping with the versant hepatitis C virus genotype assay (LiPA), version 2.0.Journal of clinical microbiology · 2013Article
- Important factors in reliable determination of hepatitis C virus genotype by use of the 5' untranslated region.Journal of clinical microbiology · 2013Article
- Evaluation of two hepatitis C virus genotyping assays based on the 5' untranslated region (UTR): the limitations of 5' UTR-based assays and the need for a supplementary sequencing-based approach.Journal of clinical microbiology · 2012Article
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Authors and funding
8 authors.
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Abstract
backgroundGenotyping of hepatitis C virus (HCV) has become an essential tool for prognosis and prediction of treatment duration. The aim of this study was to compare two HCV genotyping methods: reverse hybridization line probe assay (LiPA v.1) and partial sequencing of the NS5B region.
methodsPlasma of 171 patients with chronic hepatitis C were screened using both a commercial method (LiPA HCV Versant, Siemens, Tarrytown, NY, USA) and different primers targeting the NS5B region for PCR amplification and sequencing analysis.
resultsComparison of the HCV genotyping methods showed no difference in the classification at the genotype level. However, a total of 82/171 samples (47.9%) including misclassification, non-subtypable, discrepant and inconclusive results were not classified by LiPA at the subtype level but could be discriminated by NS5B sequencing. Of these samples, 34 samples of genotype 1a and 6 samples of genotype 1b were classified at the subtype level using sequencing of NS5B.
conclusionsSequence analysis of NS5B for genotyping HCV provides precise genotype and subtype identification and an accurate epidemiological representation of circulating viral strains.
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