ArticleCommunicative & integrative biology2011
Genetically encoded force sensors for measuring mechanical forces in proteins.
Article in Communicative & integrative biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Mechano-ID: Proximity Labeling of Mechanically Active Receptors Reveals the Mechanome and Tags Mechanically Active Cells.Journal of the American Chemical Society · 2025Article
- Förster resonance energy transfer efficiency measurements on vinculin tension sensors at focal adhesions using a simple and cost-effective setup.Journal of biomedical optics · 2023Article
- Small hand-designed convolutional neural networks outperform transfer learning in automated cell shape detection in confluent tissues.PloS one · 2023Article
- Förster resonance energy transfer efficiency of the vinculin tension sensor in cultured primary cortical neuronal growth cones.Neurophotonics · 2022Article
- Mechanical regulation of early vertebrate embryogenesis.Nature reviews. Molecular cell biology · 2022Review
- Force From Filaments: The Role of the Cytoskeleton and Extracellular Matrix in the Gating of Mechanosensitive Channels.Frontiers in cell and developmental biology · 2022Review
- Characterization of the Striatal Extracellular Matrix in a Mouse Model of Parkinson's Disease.Antioxidants (Basel, Switzerland) · 2021Article
- Biomechanics of Collective Cell Migration in Cancer Progression: Experimental and Computational Methods.ACS biomaterials science & engineering · 2019Article
- A toolbox to explore the mechanics of living embryonic tissues.Seminars in cell & developmental biology · 2016Review
- Mechanical transduction by ion channels: A cautionary tale.World journal of neurology · 2015Article
- A Threshold Shear Force for Calcium Influx in an Astrocyte Model of Traumatic Brain Injury.Journal of neurotrauma · 2015Article
- Computational mechanobiology: may the force be with you.Journal of mathematical biology · 2015Article
- Yielding elastic tethers stabilize robust cell adhesion.PLoS computational biology · 2014Article
- Shear-induced force transmission in a multicomponent, multicell model of the endothelium.Journal of the Royal Society, Interface · 2014Article
- Studying genomic processes at the single-molecule level: introducing the tools and applications.Nature reviews. Genetics · 2013Review
- E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Roles of cell confluency and fluid shear in 3-dimensional intracellular forces in endothelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Molecular force transduction by ion channels: diversity and unifying principles.Journal of cell science · 2012Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
there are three sources of free energy for cellschemical potential, electrical potential and mechanical potential. There is little known about the last one since there have not been simple ways to measure stress in proteins in cells. we have now developed genetically encoded force sensors to assess the stress in fibrous proteins in living cells. These FReT based fluorescence sensors can be read out at video rates and provide real time maps of the stress distribution in cells, tissues and animals. The sensors can be inserted into specific proteins and in general do not disturb the normal function or anatomy. The original sensors used mutant GFPs linked by elastic linkers. These sensors provide a linear output with applied stress but the response is linear in strain. To improve contrast and dynamic range we have now developed a new class of sensors that are smaller making them less invasive, and have much higher intrinsic sensitivity since force modulates the angle between the donor and acceptor much more than the distance between them. Known as cpstFRET, the probe shows improved biocompatibility, wider dynamic range and higher sensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.