ArticlePLoS computational biology2011
Filament depolymerization can explain chromosome pulling during bacterial mitosis.
Article in PLoS computational biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Genome Segregation by the Venus Flytrap Mechanism: Probing the Interaction Between the ParF ATPase and the ParG Centromere Binding Protein.Frontiers in molecular biosciences · 2020Article
- Regulation of Pom cluster dynamics in Myxococcus xanthus.PLoS computational biology · 2018Article
- Self-organization principles of intracellular pattern formation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Brownian Ratchet Mechanism for Faithful Segregation of Low-Copy-Number Plasmids.Biophysical journal · 2017Article
- DNA-relay mechanism is sufficient to explain ParA-dependent intracellular transport and patterning of single and multiple cargos.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Self-propulsion and interactions of catalytic particles in a chemically active medium.Physical review. E · 2016Article
- Directed and persistent movement arises from mechanochemistry of the ParA/ParB system.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Breaking and restoring the hydrophobic core of a centromere-binding protein.The Journal of biological chemistry · 2015Article
- Competing ParA structures space bacterial plasmids equally over the nucleoid.PLoS computational biology · 2014Article
- Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization and interactions with its partner ParB.Microbiology (Reading, England) · 2014Article
- Condensation and localization of the partitioning protein ParB on the bacterial chromosome.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Control of actin-based motility through localized actin binding.Physical biology · 2013Article
- Article
- Multidimensional view of the bacterial cytoskeleton.Journal of bacteriology · 2013Review
- Caulobacter chromosome in vivo configuration matches model predictions for a supercoiled polymer in a cell-like confinement.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The internal organization of mycobacterial partition assembly: does the DNA wrap a protein core?PloS one · 2012Article
- Cytoskeletal Proteins in Caulobacter crescentus: Spatial Orchestrators of Cell Cycle Progression, Development, and Cell Shape.Sub-cellular biochemistryReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Chromosome segregation is fundamental to all cells, but the force-generating mechanisms underlying chromosome translocation in bacteria remain mysterious. Caulobacter crescentus utilizes a depolymerization-driven process in which a ParA protein structure elongates from the new cell pole, binds to a ParB-decorated chromosome, and then retracts via disassembly, pulling the chromosome across the cell. This poses the question of how a depolymerizing structure can robustly pull the chromosome that disassembles it. We perform Brownian dynamics simulations with a simple, physically consistent model of the ParABS system. The simulations suggest that the mechanism of translocation is "self-diffusiophoretic": by disassembling ParA, ParB generates a ParA concentration gradient so that the ParA concentration is higher in front of the chromosome than behind it. Since the chromosome is attracted to ParA via ParB, it moves up the ParA gradient and across the cell. We find that translocation is most robust when ParB binds side-on to ParA filaments. In this case, robust translocation occurs over a wide parameter range and is controlled by a single dimensionless quantity: the product of the rate of ParA disassembly and a characteristic relaxation time of the chromosome. This time scale measures the time it takes for the chromosome to recover its average shape after it is has been pulled. Our results suggest explanations for observed phenomena such as segregation failure, filament-length-dependent translocation velocity, and chromosomal compaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.