ArticlePlant & cell physiology2011
Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries.
Article in Plant & cell physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers, 1 of them a synthesis that pooled it.
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Who cites it
73 citing papers in PubMed, 1 synthesis or guideline pooled it, 189 citations in OpenAlex.
- Pooled it
- Arbuscular mycorrhizal symbiosis in tomato roots with a diverse range of carotene accumulation.Mycorrhiza · 2026Article
- CpERF-WRI1 Manipulates Ethylene Sensing by Regulating the Expression of CpERS1 and Fruit Ripening in Papaya.Plant biotechnology journal · 2026Article
- Forward genetic screening of mutants of the wild diploid ancestor Nicotiana sylvestris identifies aspartate oxidase 2 as a key gene for nicotine biosynthesis in cultivated allotetraploid tobacco (Nicotiana tabacum).The Plant journal : for cell and molecular biology · 2026Article
- What metabolomics has taught us about tomato fruit ripening and quality.Journal of experimental botany · 2025Review
- Abscission zones: cellular interfaces for the programmed separation of organs.Annals of botany · 2025Review
- High throughput screening and evaluation of salt-tolerant mutants from an EMS collection ofFrontiers in plant science · 2025Article
- Development of Ac- and Ds-tagged starter lines for large-scale transposon-mutagenesis in tomato.PloS one · 2025Article
- Near-complete genome assembly of tomato (Plant biotechnology (Tokyo, Japan) · 2024Article
- Functions of membrane proteins in regulating fruit ripening and stress responses of horticultural crops.Molecular horticulture · 2024Review
- Reactive Oxygen and Related Regulatory Factors Involved in Ethylene-Induced Petal Abscission in Roses.Plants (Basel, Switzerland) · 2024Article
- Review
- Diversity of tomato leaf form provides novel insights into breeding.Breeding science · 2023Article
- Targeted system approach to ethylene biosynthesis and signaling of a heat tolerant tomato cultivar; the impact of growing season on fruit ripening.Frontiers in plant science · 2023Article
- Augmenting tomato functional genomics with a genome-wide induced genetic variation resource.Frontiers in plant science · 2023Article
- Current status, breeding strategies and future prospects for managing chilli leaf curl virus disease and associated begomoviruses in Chilli (Frontiers in plant science · 2023Review
- Is it the end of TILLING era in plant science?Frontiers in plant science · 2023Review
- Transcriptomic analysis in tomato fruit reveals divergences in genes involved in cold stress response and fruit ripening.Frontiers in plant science · 2023Article
- An overview of ethylene insensitive tomato mutants: Advantages and disadvantages for postharvest fruit shelf-life and future perspective.Frontiers in plant science · 2023Review
- Acetylome reprograming participates in the establishment of fruit metabolism during polyploidization in citrus.Plant physiology · 2022Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To accelerate functional genomic research in tomato, we developed a Micro-Tom TILLING (Targeting Induced Local Lesions In Genomes) platform. DNA pools were constructed from 3,052 ethyl methanesulfonate (EMS) mutant lines treated with 0.5 or 1.0% EMS. The mutation frequency was calculated by screening 10 genes. The 0.5% EMS population had a mild mutation frequency of one mutation per 1,710 kb, whereas the 1.0% EMS population had a frequency of one mutation per 737 kb, a frequency suitable for producing an allelic series of mutations in the target genes. The overall mutation frequency was one mutation per 1,237 kb, which affected an average of three alleles per kilobase screened. To assess whether a Micro-Tom TILLING platform could be used for efficient mutant isolation, six ethylene receptor genes in tomato (SlETR1-SlETR6) were screened. Two allelic mutants of SlETR1 (Sletr1-1 and Sletr1-2) that resulted in reduced ethylene responses were identified, indicating that our Micro-Tom TILLING platform provides a powerful tool for the rapid detection of mutations in an EMS mutant library. This work provides a practical and publicly accessible tool for the study of fruit biology and for obtaining novel genetic material that can be used to improve important agronomic traits in tomato.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.