ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2011
Mature N-linked glycans facilitate UT-A1 urea transporter lipid raft compartmentalization.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Increased plasmin-mediated proteolysis of L1CAM in a mouse model of idiopathic normal pressure hydrocephalus.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Above the Matrix: Functional Roles for Apically Localized Integrins.Frontiers in cell and developmental biology · 2021Review
- The urea transporter UT-A1 plays a predominant role in a urea-dependent urine-concentrating mechanism.The Journal of biological chemistry · 2020Article
- E3 ligase MDM2 mediates urea transporter-A1 ubiquitination under either constitutive or stimulatory conditions.American journal of physiology. Renal physiology · 2019Article
- Identification of a Novel UT-B Urea Transporter in Human Urothelial Cancer.Frontiers in physiology · 2017Article
- N-glycosylation critically regulates function of oxalate transporter SLC26A6.American journal of physiology. Cell physiology · 2016Article
- Modulation of kidney urea transporter UT-A3 activity by alpha2,6-sialylation.Pflugers Archiv : European journal of physiology · 2016Article
- Glycosylation of solute carriers: mechanisms and functional consequences.Pflugers Archiv : European journal of physiology · 2016Review
- Regulation of the Axillary Osmidrosis-Associated ABCC11 Protein Stability by N-Linked Glycosylation: Effect of Glucose Condition.PloS one · 2016Article
- Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures.Biomolecules · 2015Article
- Lovastatin-Induced Phosphatidylinositol-4-Phosphate 5-Kinase Diffusion from Microvilli Stimulates ROMK Channels.Journal of the American Society of Nephrology : JASN · 2015Article
- N-glycosylation is essential for ileal ASBT function and protection against proteases.American journal of physiology. Cell physiology · 2015Article
- Activation of protein kinase C-α and Src kinase increases urea transporter A1 α-2, 6 sialylation.Journal of the American Society of Nephrology : JASN · 2015Article
- Dexamethasone increases aquaporin-2 protein expression in ex vivo inner medullary collecting duct suspensions.Frontiers in physiology · 2015Article
- Solenopsin A and analogs exhibit ceramide-like biological activity.Vascular cell · 2015Article
- RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.Frontiers in physiology · 2015Article
- Activation of the cAMP/PKA pathway induces UT-A1 urea transporter monoubiquitination and targets it for lysosomal degradation.American journal of physiology. Renal physiology · 2013Article
- Green tea phenolics inhibit butyrate-induced differentiation of colon cancer cells by interacting with monocarboxylate transporter 1.Biochimica et biophysica acta · 2013Article
- Small GTPase Rab14 down-regulates UT-A1 urea transport activity through enhanced clathrin-dependent endocytosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- New advances in urea transporter UT-A1 membrane trafficking.International journal of molecular sciences · 2013Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The UT-A1 urea transporter is a glycoprotein with two different glycosylated forms of 97 and 117 kDa. In this study, we found the 117-kDa UT-A1 preferentially resides in lipid rafts, suggesting that the glycosylation status may interfere with UT-A1 lipid raft trafficking. This was confirmed by a site-directed mutagenesis study in MDCK cells. The nonglycosylated UT-A1 showed reduced localization in lipid rafts. By using sugar-specific binding lectins, we further found that the UT-A1 in nonlipid rafts contained a high amount of mannose, as detected by concanavalin A, while the UT-A1 in lipid rafts was the mature N-acetylglucosamine-containing form, as detected by wheat germ agglutinin. In the inner medulla (IM) of diabetic rats, the more abundant 117-kDa UT-A1 in lipid rafts was the mature glycosylation form, with high amounts of N-acetylglucosamine and sialic acid. In contrast, in the IM of normal rats, the predominant 97-kDa UT-A1 was the form enriched in mannose. Functionally, inhibition of glycosylation by tunicamycin or elimination of the glycosylation sites by mutation significantly reduced UT-A1 activity in oocytes. Taken together, our study reveals a new role of N-linked glycosylation in regulating UT-A1 activity by promoting UT-A1 trafficking into membrane lipid raft subdomains.
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