ArticleJournal of bacteriology2011
Hopanoid production is required for low-pH tolerance, antimicrobial resistance, and motility in Burkholderia cenocepacia.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 119 citations in OpenAlex.
- The phospholipase PlcP mediates membrane lipid remodeling required for the intracellular survival of Burkholderia cenocepacia.The Journal of biological chemistry · 2026Article
- Horizontal acquisition of prokaryotic hopanoid biosynthesis reorganizes membrane physiology driving lifestyle innovation in a eukaryote.Nature communications · 2025Article
- Methanotroph-methylotroph lipid adaptations to changing environmental conditions.Frontiers in microbiology · 2025Article
- Boreal moss-microbe interactions are revealed through metagenome assembly of novel bacterial species.Scientific reports · 2024Article
- Hopanoid lipids promote soybeanmBio · 2024Article
- A Mildly Acidic Environment Alters Pseudomonas aeruginosa Virulence and Causes Remodeling of the Bacterial Surface.Microbiology spectrum · 2023Article
- Lipid Transport Across Bacterial Membranes.Annual review of cell and developmental biology · 2022Review
- Genome mining of Burkholderia ambifaria strain T16, a rhizobacterium able to produce antimicrobial compounds and degrade the mycotoxin fusaric acid.World journal of microbiology & biotechnology · 2022Article
- Lipid biomarkers: molecular tools for illuminating the history of microbial life.Nature reviews. Microbiology · 2022Review
- System-Level Analysis of Transcriptional and Translational Regulatory Elements inFrontiers in bioengineering and biotechnology · 2022Article
- Metabolic Profiling of Interspecies Interactions During Sessile Bacterial Cultivation Reveals Growth and Sporulation Induction inFrontiers in cellular and infection microbiology · 2022Article
- Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors.Scientific reports · 2021Article
- A squalene-hopene cyclase inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- A link between pH homeostasis and colistin resistance in bacteria.Scientific reports · 2021Article
- Structural and Functional Diversity of Resistance-Nodulation-Cell Division Transporters.Chemical reviews · 2021Review
- The LactococcalInternational journal of molecular sciences · 2021Article
- Genome-Based Characterization of Plant-AssociatedFrontiers in microbiology · 2021Article
- The antimicrobial peptide Polybia-MP1 differentiates membranes with the hopanoid, diplopterol from those with cholesterol.BBA advances · 2021Article
- A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis.Applied and environmental microbiology · 2020Article
- Introduction of a green algal squalene synthase enhances squalene accumulation in a strain ofMetabolic engineering communications · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hopanoids are pentacyclic triterpenoids that are thought to be bacterial surrogates for eukaryotic sterols, such as cholesterol, acting to stabilize membranes and to regulate their fluidity and permeability. To date, very few studies have evaluated the role of hopanoids in bacterial physiology. The synthesis of hopanoids depends on the enzyme squalene-hopene cyclase (Shc), which converts the linear squalene into the basic hopene structure. Deletion of the 2 genes encoding Shc enzymes in Burkholderia cenocepacia K56-2, BCAM2831 and BCAS0167, resulted in a strain that was unable to produce hopanoids, as demonstrated by gas chromatography and mass spectrometry. Complementation of the Δshc mutant with only BCAM2831 was sufficient to restore hopanoid production to wild-type levels, while introducing a copy of BCAS0167 alone into the Δshc mutant produced only very small amounts of the hopanoid peak. The Δshc mutant grew as well as the wild type in medium buffered to pH 7 and demonstrated no defect in its ability to survive and replicate within macrophages, despite transmission electron microscopy (TEM) revealing defects in the organization of the cell envelope. The Δshc mutant displayed increased sensitivity to low pH, detergent, and various antibiotics, including polymyxin B and erythromycin. Loss of hopanoid production also resulted in severe defects in both swimming and swarming motility. This suggests that hopanoid production plays an important role in the physiology of B. cenocepacia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.