ArticleJournal of bacteriology2011
Bacteriophage F336 recognizes the capsular phosphoramidate modification of Campylobacter jejuni NCTC11168.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed, 115 citations in OpenAlex.
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- Campylobacteriosis and Control Strategies againstJournal of microbiology and biotechnology · 2024Review
- Bacteriophages from human skin infecting coagulase-negative Staphylococcus: diversity, novelty and host resistance.Scientific reports · 2024Article
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- Defense and anti-defense mechanisms of bacteria and bacteriophages.Journal of Zhejiang University. Science. B · 2024Article
- A hybrid receptor binding protein enables phage F341 infection ofFrontiers in microbiology · 2024Article
- Bacteriophage cocktail application for Campylobacter mitigation - from in vitro to in vivo.BMC microbiology · 2023Article
- Identification of Capsular Polysaccharide Synthesis Loci Determining Bacteriophage Susceptibility in Tetragenococcus halophilus.Microbiology spectrum · 2023Article
- Phage Adsorption to Gram-Positive Bacteria.Viruses · 2023Review
- Characterizations of novel broad-spectrum lytic bacteriophagesFrontiers in microbiology · 2023Article
- Bacteriophages as Biocontrol Agents in Livestock Food Production.Microorganisms · 2022Review
- Evolutionary Dynamics between Phages and Bacteria as a Possible Approach for Designing Effective Phage Therapies against Antibiotic-Resistant Bacteria.Antibiotics (Basel, Switzerland) · 2022Review
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- APTC-EC-2A: A Lytic Phage Targeting Multidrug ResistantMicroorganisms · 2022Article
- Bacterial glycosylation, it's complicated.Frontiers in molecular biosciences · 2022Review
- Colistin-phage combinations decrease antibiotic resistance inEmerging microbes & infections · 2021Article
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- In vitro efficiency evaluation of phage cocktail for biocontrol of Salmonella spp. in food products.Archives of microbiology · 2021Article
- Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteriophages infecting the food-borne human pathogen Campylobacter jejuni could potentially be exploited to reduce bacterial counts in poultry prior to slaughter. This bacterium colonizes the intestinal tract of poultry in high numbers, and contaminated poultry meat is regarded as the major source of human campylobacteriosis. In this study, we used phage F336 belonging to the Myoviridae family to select a C. jejuni NCTC11168 phage-resistant strain, called 11168R, with the aim of investigating the mechanisms of phage resistance. We found that phage F336 has reduced adsorption to 11168R, thus indicating that the receptor is altered. While proteinase K-treated C. jejuni cells did not affect adsorption, periodate treatment resulted in reduced adsorption, suggesting that the phage binds to a carbohydrate moiety. Using high-resolution magic angle spinning nuclear magnetic resonance (NMR) spectroscopy, we found that 11168R lacks an O-methyl phosphoramidate (MeOPN) moiety attached to the GalfNAc on the capsular polysaccharide (CPS), which was further confirmed by mass spectroscopy. Sequence analysis of 11168R showed that the potentially hypervariable gene cj1421, which encodes the GalfNAc MeOPN transferase, contains a tract of 10 Gs, resulting in a nonfunctional gene product. However, when 11168R reverted back to phage sensitive, cj1421 contained 9 Gs, and the GalfNAc MeOPN was regained in this strain. In summary, we have identified the phase-variable MeOPN moiety, a common component of the diverse capsular polysaccharides of C. jejuni, as a novel receptor of phages infecting this bacterium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.