ArticleApplied and environmental microbiology2011
High ethanol titers from cellulose by using metabolically engineered thermophilic, anaerobic microbes.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 119 papers.
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Who cites it
119 citing papers in PubMed.
- Diverse thermophilic anaerobes from Indian hot springs show potential for bioenergy production from lignocellulosic biomass.World journal of microbiology & biotechnology · 2026Article
- Grey-box modeling framework for consolidated bioprocessing systems: an endpoint-guided approach.Bioresources and bioprocessing · 2026Article
- EngineeringMetabolic engineering communications · 2026Article
- Genetic and metabolic drivers of membrane remodeling inmSystems · 2026Article
- Research progress in plant endophyte-mediated lignocellulosic biomass degradation and valorization: a review.Archives of microbiology · 2026Review
- EngineeringbioRxiv : the preprint server for biology · 2025Article
- Designing thermophilic, synthetic microbial communities for consolidated bioprocessing.Biodesign research · 2025Review
- Thermophilic site-specific recombination system for rapid insertion of heterologous DNA into the Clostridium thermocellum chromosome.Journal of industrial microbiology & biotechnology · 2024Article
- Expression and characterization of monofunctional alcohol dehydrogenase enzymes inMetabolic engineering communications · 2024Article
- Maximizing microbial activity and synergistic interaction to boost biofuel production from lignocellulosic biomass.Archives of microbiology · 2024Review
- The role of AdhE on ethanol tolerance and production in Clostridium thermocellum.The Journal of biological chemistry · 2024Article
- Research advances on the consolidated bioprocessing of lignocellulosic biomass.Engineering microbiology · 2024Review
- Extremophiles in a changing world.Extremophiles : life under extreme conditions · 2024Review
- Ethanol tolerance in engineered strains of Clostridium thermocellum.Biotechnology for biofuels and bioproducts · 2023Article
- Metabolic engineering of Thermoanaerobacterium AK17 for increased ethanol production in seaweed hydrolysate.Biotechnology for biofuels and bioproducts · 2023Article
- Efficient production of lactic acid from cellulose and xylan in sugarcane bagasse by newly isolatedHeliyon · 2023Article
- Characterization and Amelioration of Filtration Difficulties Encountered in Metabolomic Studies of Clostridium thermocellum at Elevated Sugar Concentrations.Applied and environmental microbiology · 2023Article
- Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing.Microbial biotechnology · 2023Review
- The Roles of Nicotinamide Adenine Dinucleotide Phosphate Reoxidation and Ammonium Assimilation in the Secretion of Amino Acids as Byproducts of Clostridium thermocellum.Applied and environmental microbiology · 2023Article
- Direct conversion of cellulose to L-lactic acid by a novel thermophilic Caldicellulosiruptor strain.Biotechnology for biofuels and bioproducts · 2022Article
59 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This work describes novel genetic tools for use in Clostridium thermocellum that allow creation of unmarked mutations while using a replicating plasmid. The strategy employed counter-selections developed from the native C. thermocellum hpt gene and the Thermoanaerobacterium saccharolyticum tdk gene and was used to delete the genes for both lactate dehydrogenase (Ldh) and phosphotransacetylase (Pta). The Δldh Δpta mutant was evolved for 2,000 h, resulting in a stable strain with 40:1 ethanol selectivity and a 4.2-fold increase in ethanol yield over the wild-type strain. Ethanol production from cellulose was investigated with an engineered coculture of organic acid-deficient engineered strains of both C. thermocellum and T. saccharolyticum. Fermentation of 92 g/liter Avicel by this coculture resulted in 38 g/liter ethanol, with acetic and lactic acids below detection limits, in 146 h. These results demonstrate that ethanol production by thermophilic, cellulolytic microbes is amenable to substantial improvement by metabolic engineering.
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