ArticleApplied and environmental microbiology2011
National validation study of a cellulose sponge wipe-processing method for use after sampling Bacillus anthracis spores from surfaces.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Comparison of Two Glove-Sampling Methods to Discriminate Between Study Arms of a Hand Hygiene and Glove-Use Study.Infection control and hospital epidemiology · 2018Trial
- Methods for detecting highly pathogenic avian influenza H5N1 virus in dairy processing environments.Frontiers in microbiology · 2026Article
- Comparison of sampling and culture methods for the recovery of yeast from hospital surfaces.Antimicrobial stewardship & healthcare epidemiology : ASHE · 2025Article
- Fate and transport of viableJournal of the Air & Waste Management Association (1995) · 2024Article
- Evaluation of sponge wipe surface sampling for collection of potential surrogates for non-spore-forming bioterrorism agents.Journal of applied microbiology · 2024Article
- Investigating surface area and recovery efficiency of healthcare-associated pathogens to optimize composite environmental sampling.PloS one · 2024Article
- Sampling and recovery of infectious SARS-CoV-2 from high-touch surfaces by sponge stick and macrofoam swab.Journal of occupational and environmental hygiene · 2023Article
- Passenger vehicle interior decontamination by low concentration hydrogen peroxide vapor following a wide area biological contamination incident.Journal of applied microbiology · 2023Article
- Potential sources of contamination on textiles and hard surfaces identified as high-touch sites near the patient environment.PloS one · 2023Article
- Evaluation of sample processing methods to improve the detection of Bacillus anthracis in difficult sample matrices.Environmental monitoring and assessment · 2022Article
- Epidemiologic Investigation of Two Welder's Anthrax Cases Caused byPathogens (Basel, Switzerland) · 2022Article
- Environmental Contamination of Contact Precaution and Non-Contact Precaution Patient Rooms in Six Acute Care Facilities.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Comparison of surface sampling methods for an extended duration outdoor biological contamination study.Environmental monitoring and assessment · 2020Article
- A microbiological survey of handwashing sinks in the hospital built environment reveals differences in patient room and healthcare personnel sinks.Scientific reports · 2020Article
- Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR.BMC microbiology · 2020Article
- Development of a rapid-viability PCR method for detection of Clostridioides difficile spores from environmental samples.Anaerobe · 2020Article
- Elution efficiency of healthcare pathogens from environmental sampling tools.Infection control and hospital epidemiology · 2020Article
- Rapid viability polymerase chain reaction method for detection of Francisella tularensis.Journal of microbiological methods · 2019Article
- Preventing Viral Contamination: Effects of Wipe and Spray-based Decontamination of Gloves and Gowns.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Effect of Glove Decontamination on Bacterial Contamination of Healthcare Personnel Hands.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
Corrections and comments
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This work was initiated to address the gaps identified by Congress regarding validated biothreat environmental sampling and processing methods. Nine Laboratory Response Network-affiliated laboratories participated in a validation study of a cellulose sponge wipe-processing protocol for the recovery, detection, and quantification of viable Bacillus anthracis Sterne spores from steel surfaces. Steel coupons (645.16 cm(2)) were inoculated with 1 to 4 log(10) spores and then sampled with cellulose sponges (Sponge-Stick; 3M, St. Paul, MN). Surrogate dust and background organisms were added to the sponges to mimic environmental conditions. Labs processed the sponges according to the provided protocol. Sensitivity, specificity, and mean percent recovery (%R), between-lab variability, within-lab variability, and total percent coefficient of variation were calculated. The mean %R (standard error) of spores from the surface was 32.4 (4.4), 24.4 (2.8), and 30.1 (2.3) for the 1-, 2-, and 4-log(10) inoculum levels, respectively. Sensitivities for colony counts were 84.1%, 100%, and 100% for the 1-, 2-, and 4-log(10) inocula, respectively. These data help to characterize the variability of the processing method and thereby enhance confidence in the interpretation of the results of environmental sampling conducted during a B. anthracis contamination investigation.
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