ArticleApplied and environmental microbiology2011
Combined effect of improved cell yield and increased specific productivity enhances recombinant enzyme production in genome-reduced Bacillus subtilis strain MGB874.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 76 citations in OpenAlex.
- Toward Antibody Production in Genome-MinimizedACS synthetic biology · 2025Article
- Metabolic Profile of the Genome-ReducedACS synthetic biology · 2024Article
- Enhanced protein secretion in reduced genome strains of Streptomyces lividans.Microbial cell factories · 2024Article
- Let There Be Light: Genome Reduction EnablesACS synthetic biology · 2023Article
- Greedy reduction of Bacillus subtilis genome yields emergent phenotypes of high resistance to a DNA damaging agent and low evolvability.Nucleic acids research · 2023Article
- Engineering the gut microbiota to treat chronic diseases.Applied microbiology and biotechnology · 2020Review
- Metabolic engineering ofSynthetic and systems biotechnology · 2019Article
- Development of a secretory expression system with high compatibility between expression elements and an optimized host for endoxylanase production in Corynebacterium glutamicum.Microbial cell factories · 2019Article
- Engineering and modification of microbial chassis for systems and synthetic biology.Synthetic and systems biotechnology · 2019Review
- Less Is More: Toward a Genome-Reduced Bacillus Cell Factory for "Difficult Proteins".ACS synthetic biology · 2019Article
- The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment.Polish journal of microbiology · 2019Article
- Poly-L-gamma-glutamic acid production by recombinant Bacillus subtilis without pgsA gene.AMB Express · 2018Article
- The contribution of bacterial genome engineering to sustainable development.Microbial biotechnology · 2017Article
- Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine.Microbial cell factories · 2016Article
- Updates on industrial production of amino acids using Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2016Review
- Deletion of genes involved in glutamate metabolism to improve poly-gamma-glutamic acid production in B. amyloliquefaciens LL3.Journal of industrial microbiology & biotechnology · 2015Article
- Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 for use as a platform strain for basic research and industrial biotechnology.Applied and environmental microbiology · 2013Article
- Characterization of genome-reduced fission yeast strains.Nucleic acids research · 2013Article
- Improved production of secreted heterologous enzyme in Bacillus subtilis strain MGB874 via modification of glutamate metabolism and growth conditions.Microbial cell factories · 2013Article
- Bacillus subtilis: from soil bacterium to super-secreting cell factory.Microbial cell factories · 2013Article
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10 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Genome reduction strategies to create genetically improved cellular biosynthesis machineries for proteins and other products have been pursued by use of a wide range of bacteria. We reported previously that the novel Bacillus subtilis strain MGB874, which was derived from strain 168 and has a total genomic deletion of 874 kb (20.7%), exhibits enhanced production of recombinant enzymes. However, it was not clear how the genomic reduction resulted in elevated enzyme production. Here we report that deletion of the rocDEF-rocR region, which is involved in arginine degradation, contributes to enhanced enzyme production in strain MGB874. Deletion of the rocDEF-rocR region caused drastic changes in glutamate metabolism, leading to improved cell yields with maintenance of enzyme productivity. Notably, the specific enzyme productivity was higher in the reduced-genome strain, with or without the rocDEF-rocR region, than in wild-type strain 168. The high specific productivity in strain MGB874 is likely attributable to the higher expression levels of the target gene resulting from an increased promoter activity and plasmid copy number. Thus, the combined effects of the improved cell yield by deletion of the rocDEF-rocR region and the increased specific productivity by deletion of another gene(s) or the genomic reduction itself enhanced the production of recombinant enzymes in MGB874. Our findings represent a good starting point for the further improvement of B. subtilis reduced-genome strains as cell factories for the production of heterologous enzymes.
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