ArticleMolecular biology of the cell2011
Cx50 requires an intact PDZ-binding motif and ZO-1 for the formation of functional intercellular channels.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 31 citations in OpenAlex.
- N-acetylglucosaminyltransferase V drives colorectal cancer metastasis by facilitating ZO-1 ubiquitination and degradation.Cancer cell international · 2024Article
- Dual diagnosis ofRare (Amsterdam, Netherlands) · 2024Article
- Charged multivesicular body protein 4b forms complexes with gap junction proteins during lens fiber cell differentiation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- The IgSF Cell Adhesion Protein CLMP and Congenital Short Bowel Syndrome (CSBS).International journal of molecular sciences · 2023Review
- Biochemical and biomechanical characteristics of dystrophin-deficient mdxBiochimica et biophysica acta. Molecular basis of disease · 2021Article
- MicroRNA‑103 regulates tumorigenesis in colorectal cancer by targeting ZO‑1.Molecular medicine reports · 2018Article
- Focus on lens connexins.BMC cell biology · 2017Review
- A novel frameshift mutation in CX46 associated with hereditary dominant cataracts in a Chinese family.International journal of ophthalmology · 2017Article
- Knock-in of Cx46 partially rescues fiber defects in lenses lacking Cx50.Molecular vision · 2017Article
- Identification and Functional Assessment of Age-Dependent Truncations to Cx46 and Cx50 in the Human Lens.Investigative ophthalmology & visual science · 2016Article
- Connexin43 hemichannels contributes to the disassembly of cell junctions through modulation of intracellular oxidative status.Redox biology · 2016Article
- Connexin 50 Regulates Surface Ball-and-Socket Structures and Fiber Cell Organization.Investigative ophthalmology & visual science · 2016Article
- Differential regulation of Connexin50 and Connexin46 by PI3K signaling.FEBS letters · 2015Article
- The carboxyl tail of connexin32 regulates gap junction assembly in human prostate and pancreatic cancer cells.The Journal of biological chemistry · 2015Article
- AII amacrine cells discriminate between heterocellular and homocellular locations when assembling connexin36-containing gap junctions.Journal of cell science · 2014Article
- A connexin50 mutant, CX50fs, that causes cataracts is unstable, but is rescued by a proteasomal inhibitor.The Journal of biological chemistry · 2013Article
- Proteins and mechanisms regulating gap-junction assembly, internalization, and degradation.Physiology (Bethesda, Md.) · 2013Review
- The role of the C-terminus in functional expression and internalization of rat connexin46 (rCx46).Journal of bioenergetics and biomembranes · 2013Article
- Upregulated ZO-1 correlates with favorable survival of gastrointestinal stromal tumor.Medical oncology (Northwood, London, England) · 2013Article
- A cataract-causing connexin 50 mutant is mislocalized to the ER due to loss of the fourth transmembrane domain and cytoplasmic domain.FEBS open bio · 2013Article
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3 authors at 1 institution in 1 country.
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Abstract
The three connexins expressed in the ocular lens each contain PDZ domain-binding motifs directing a physical association with the scaffolding protein ZO-1, but the significance of the interaction is unknown. We found that Cx50 with PDZ-binding motif mutations did not form gap junction plaques or induce cell-cell communication in HeLa cells, whereas the addition of a seven-amino acid PDZ-binding motif restored normal function to Cx50 lacking its entire C-terminal cytoplasmic domain. C-Terminal deletion had a similar although weaker effect on Cx46 but little if any effect on targeting and function of Cx43. Furthermore, small interfering RNA knockdown of ZO-1 completely inhibited the formation of gap junctions by wild-type Cx50 in HeLa cells. Thus both a PDZ-binding motif and ZO-1 are necessary for Cx50 intercellular channel formation in HeLa cells. Knock-in mice expressing Cx50 with a PDZ-binding motif mutation phenocopied Cx50 knockouts. Furthermore, differentiating lens fibers in the knock-in displayed extensive intracellular Cx50, whereas plaques in mature fibers contained only Cx46. Thus normal Cx50 function in vivo also requires an intact PDZ domain-binding motif. This is the first demonstration of a connexin-specific requirement for a connexin-interacting protein in gap junction assembly.
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