ArticleMolecular biology of the cell2011
Subunit organization in the Dam1 kinetochore complex and its ring around microtubules.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Computed structures of core eukaryotic protein complexes.Science (New York, N.Y.) · 2021Article
- Cell-cycle phospho-regulation of the kinetochore.Current genetics · 2021Review
- Structural view of the yeast Dam1 complex, a ring-shaped molecular coupler for the dynamic microtubule end.Essays in biochemistry · 2020Review
- Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.Science (New York, N.Y.) · 2018Article
- Structural plasticity of the living kinetochore.The Journal of cell biology · 2017Article
- Article
- A Molecular View of Kinetochore Assembly and Function.Biology · 2017Review
- Article
- Molecular architecture of the Dam1 complex-microtubule interaction.Open biology · 2016Article
- The molecular architecture of the Dam1 kinetochore complex is defined by cross-linking based structural modelling.Nature communications · 2015Article
- The kinetochore encodes a mechanical switch to disrupt spindle assembly checkpoint signalling.Nature cell biology · 2015Article
- Kinetochore-microtubule error correction is driven by differentially regulated interaction modes.Nature cell biology · 2015Article
- Assembling the protein architecture of the budding yeast kinetochore-microtubule attachment using FRET.Current biology : CB · 2014Article
- Phosphoregulation promotes release of kinetochores from dynamic microtubules via multiple mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Molecular requirements for the formation of a kinetochore-microtubule interface by Dam1 and Ndc80 complexes.The Journal of cell biology · 2013Article
- Structural insights into WHAMM-mediated cytoskeletal coordination during membrane remodeling.The Journal of cell biology · 2012Article
- Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast.Journal of cell science · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
All eukaryotic cells must segregate their chromosomes equally between two daughter cells at each division. This process needs to be robust, as errors in the form of loss or gain of genetic material have catastrophic effects on viability. Chromosomes are captured, aligned, and segregated to daughter cells via interaction with spindle microtubules mediated by the kinetochore. In Saccharomyces cerevisiae one microtubule attaches to each kinetochore, requiring extreme processivity from this single connection. The yeast Dam1 complex, an essential component of the outer kinetochore, forms rings around microtubules and in vitro recapitulates much of the functionality of a kinetochore-microtubule attachment. To understand the mechanism of the Dam1 complex at the kinetochore, we must know how it binds to microtubules, how it assembles into rings, and how assembly is regulated. We used electron microscopy to map several subunits within the structure of the Dam1 complex and identify the organization of Dam1 complexes within the ring. Of importance, new data strongly support a more passive role for the microtubule in Dam1 ring formation. Integrating this information with previously published data, we generated a structural model for the Dam1 complex assembly that advances our understanding of its function and will direct future experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.