ArticleAmerican journal of physiology. Lung cellular and molecular physiology2012
A role for two-pore K⁺ channels in modulating Na⁺ absorption and Cl⁻ secretion in normal human bronchial epithelial cells.
Article in American journal of physiology. Lung cellular and molecular physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- A genome-wide analysis of the response to inhaled β2-agonists in chronic obstructive pulmonary disease.The pharmacogenomics journal · 2016Pooled it
- T2R38 taste receptor polymorphisms underlie susceptibility to upper respiratory infection.The Journal of clinical investigation · 2012Trial
- Potassium Channels of the Airway Epithelium.Acta physiologica (Oxford, England) · 2026Review
- Associations Between Preoperative Shortness of Breath and Potassium Channels Gene Variations in Women With Breast Cancer.Biological research for nursing · 2025Article
- The role of potassium ion channels in chronic sinusitis.Frontiers in pharmacology · 2024Review
- Physiology and pathophysiology of human airway mucus.Physiological reviews · 2022Review
- Genome-wide DNA methylation analysis of pulmonary function in middle and old-aged Chinese monozygotic twins.Respiratory research · 2021Article
- Bitter taste receptor agonists regulate epithelial two-pore potassium channels via cAMP signaling.Respiratory research · 2021Article
- An Intriguing Involvement of Mitochondria in Cystic Fibrosis.Journal of clinical medicine · 2019Review
- P2Y receptor regulation of K2P channels that facilitate KAmerican journal of physiology. Cell physiology · 2018Article
- A New Role for the Mitochondrial Pro-apoptotic Protein SMAC/Diablo in Phospholipid Synthesis Associated with Tumorigenesis.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Article
- Gene Expression Correlated with Severe Asthma Characteristics Reveals Heterogeneous Mechanisms of Severe Disease.American journal of respiratory and critical care medicine · 2017Article
- Differentiation of human bronchial epithelial cells: role of hydrocortisone in development of ion transport pathways involved in mucociliary clearance.American journal of physiology. Cell physiology · 2016Article
- Article
- Much more than a leak: structure and function of K₂p-channels.Pflugers Archiv : European journal of physiology · 2015Review
- Pulmonary epithelial barrier function: some new players and mechanisms.American journal of physiology. Lung cellular and molecular physiology · 2015Review
- Potassium channels in pancreatic duct epithelial cells: their role, function and pathophysiological relevance.Pflugers Archiv : European journal of physiology · 2015Review
- CFTR and lung homeostasis.American journal of physiology. Lung cellular and molecular physiology · 2014Review
- Deficiency of the two-pore-domain potassium channel TREK-1 promotes hyperoxia-induced lung injury.Critical care medicine · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Mucociliary clearance is the primary innate physical defense mechanism against inhaled pathogens and toxins. Vectorial ion transport, primarily sodium absorption and anion secretion, by airway epithelial cells supports mucociliary clearance. This is evidenced by diseases of abnormal ion transport such as cystic fibrosis and pseudohypoaldosteronism that are characterized by changes in mucociliary clearance. Sodium absorption and chloride secretion in human bronchial epithelial cells depend on potassium channel activity, which creates a favorable electrochemical gradient for both by hyperpolarizing the apical plasma membrane. Although the role of basolateral membrane potassium channels is firmly established and extensively studied, a role for apical membrane potassium channels has also been described. Here, we demonstrate that bupivacaine and quinidine, blockers of four-transmembrane domain, two-pore potassium (K2P) channels, inhibit both amiloride-sensitive sodium absorption and forskolin-stimulated anion secretion in polarized, normal human bronchial epithelial cells at lower concentrations when applied to the mucosal surface than when applied to the serosal surface. Transcripts from four genes, KCNK1 (TWIK-1), KCNK2 (TREK-1), KCNK5 (TASK-2), and KCNK6 (TWIK-2), encoding K2P channels were identified by RT-PCR. Protein expression at the apical membrane was confirmed by immunofluorescence. Our data provide further evidence that potassium channels, in particular K2P channels, are expressed and functional in the apical membrane of airway epithelial cells where they may be targets for therapeutic manipulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.