Evidence map›Paper›PMID 21964394›Full record

ReviewMethods (San Diego, Calif.)2011

Structure determination of membrane proteins in five easy pieces.

Francesca M Marassi, Bibhuti B Das, George J Lu, Henry J Nothnagel, Sang Ho Park, Woo Sung Son, Ye Tian, Stanley J Opella

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Methods (San Diego, Calif.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Review
  2. Membrane protein structure from rotational diffusion.Biochimica et biophysica acta · 2015
    Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Helical membrane protein conformations and their environment.European biophysics journal : EBJ · 2013
    Review
  8. Membrane protein structure determination in membrana.Accounts of chemical research · 2013
    Article
  9. Review
  10. Article
  11. Structure determination of membrane proteins by nuclear magnetic resonance spectroscopy.Annual review of analytical chemistry (Palo Alto, Calif.) · 2013
    Review
  12. Membrane protein structure determination: back to the membrane.Methods in molecular biology (Clifton, N.J.) · 2013
    Article
  13. Article
  14. Review
  15. Structure determination of a membrane protein in proteoliposomes.Journal of the American Chemical Society · 2012
    Article
  16. Article
  17. Review
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Francesca M MarassiSanford Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
Bibhuti B Das
George J Lu
Henry J Nothnagel
Sang Ho Park
Woo Sung Son
Ye Tian
Stanley J Opella

Funding

RESOURCE FOR SOLID STATE NMR OF PROTEINSP41EB002031 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2003 to 2018
$16.3M
Molecular Imaging of G-Protein-Coupled Receptors for Drug DevelopmentR01EB005161 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2006 to 2014
$6.8M
Membrane Protein Structures:Phospholipid Bilayers (RMI)R01GM075877 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2005 to 2009
$1.4M
Computational methods for NMR structure determination of proteins in membranesR21GM094727 · NIGMS · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI MARASSI, FRANCESCA M · 2010 to 2011
$509k
Structure of- beta barrel membrane protein-lipid bi(RMI)R21GM075917 · NIGMS · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI MARASSI, FRANCESCA M · 2005 to 2006
$474k
NIBIB NIH HHS P41 EB002031NIBIB NIH HHS R01 EB005161NIGMS NIH HHS R01 GM075877NIGMS NIH HHS R21 GM075917NIGMS NIH HHS R21 GM094727
6 · The paper itself

Abstract

Rotational Alignment (RA) solid-state NMR provides the basis for a general method for determining the structures of membrane proteins in phospholipid bilayers under physiological conditions. Membrane proteins are high priority targets for structure determination, and are challenging for existing experimental methods. Because membrane proteins reside in liquid crystalline phospholipid bilayer membranes it is important to study them in this type of environment. The RA solid-state NMR approach we have developed can be summarized in five steps, and incorporates methods of molecular biology, biochemistry, sample preparation, the implementation of NMR experiments, and structure calculations. It relies on solid-state NMR spectroscopy to obtain high-resolution spectra and residue-specific structural restraints for membrane proteins that undergo rotational diffusion around the membrane normal, but whose mobility is otherwise restricted by interactions with the membrane phospholipids. High resolution spectra of membrane proteins alone and in complex with other proteins and ligands set the stage for structure determination and functional studies of these proteins in their native, functional environment.

Indexed as

AnimalsBacterial ProteinsCation Transport ProteinsHumansLiposomesMembrane ProteinsNuclear Magnetic Resonance, BiomolecularProtein Structure, TertiaryReceptors, Interleukin-8ABacterial ProteinsCation Transport ProteinsLiposomesMembrane Proteinsmercury transport protein, bacterialReceptors, Interleukin-8A

Identifiers

PMID21964394
PMCPMC3264820

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.