ArticleMolecular and biochemical parasitology2012
Nuclear actin-related protein is required for chromosome segregation in Toxoplasma gondii.
Article in Molecular and biochemical parasitology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Restriction Checkpoint Controls Bradyzoite Development in Toxoplasma gondii.Microbiology spectrum · 2022Article
- Article
- The Modular Circuitry of Apicomplexan Cell Division Plasticity.Frontiers in cellular and infection microbiology · 2021Review
- A Homolog of Structural Maintenance of Chromosome 1 Is a Persistent Centromeric Protein Which Associates With Nuclear Pore Components inFrontiers in cellular and infection microbiology · 2020Article
- Apicomplexa Cell Cycles: Something Old, Borrowed, Lost, and New.Trends in parasitology · 2018Review
- Transcriptional repression by ApiAP2 factors is central to chronic toxoplasmosis.PLoS pathogens · 2018Article
- ThemBio · 2017Article
- Checkpoints of apicomplexan cell division identified in Toxoplasma gondii.PLoS pathogens · 2017Article
- Article
- A novel bipartite centrosome coordinates the apicomplexan cell cycle.PLoS biology · 2015Article
- Cell division in apicomplexan parasites.Nature reviews. Microbiology · 2014Review
- The Toxoplasma gondii kinetochore is required for centrosome association with the centrocone (spindle pole).Cellular microbiology · 2014Article
- A nucleolar AAA-NTPase is required for parasite division.Molecular microbiology · 2013Article
- Discovery of a splicing regulator required for cell cycle progression.PLoS genetics · 2013Article
- Molecular characterization of Toxoplasma gondii formin 3, an actin nucleator dispensable for tachyzoite growth and motility.Eukaryotic cell · 2012Article
- Cytoskeleton assembly in Toxoplasma gondii cell division.International review of cell and molecular biology · 2012Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Apicomplexa parasites use complex cell cycles to replicate that are not well understood mechanistically. We have established a robust forward genetic strategy to identify the essential components of parasite cell division. Here we describe a novel temperature sensitive Toxoplasma strain, mutant 13-20C2, which growth arrests due to a defect in mitosis. The primary phenotype is the mis-segregation of duplicated chromosomes with chromosome loss during nuclear division. This defect is conditional-lethal with respect to temperature, although relatively mild in regard to the preservation of the major microtubule organizing centers. Despite severe DNA loss many of the physical structures associated with daughter budding and the assembly of invasion structures formed and operated normally at the non-permissive temperature before completely arresting. These results suggest there are coordinating mechanisms that govern the timing of these events in the parasite cell cycle. The defect in mutant 13-20C2 was mapped by genetic complementation to Toxoplasma chromosome III and to a specific mutation in the gene encoding an ortholog of nuclear actin-related protein 4. A change in a conserved isoleucine to threonine in the helical structure of this nuclear actin related protein leads to protein instability and cellular mis-localization at the higher temperature. Given the age of this protist family, the results indicate a key role for nuclear actin-related proteins in chromosome segregation was established very early in the evolution of eukaryotes.
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