ArticlePlant methods2011
A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes.
Article in Plant methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 477 papers.
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Who cites it
477 citing papers in PubMed, 1,021 citations in OpenAlex.
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- A Thermo-Sensitive OsMYC2 Allele Coordinates Spikelet Development With Temperature via Proteasome-Mediated Protein Stability.Plant, cell & environment · 2026Article
- A β-1,3-glucanase antagonizes a phase-separating WRKY repressor to confer saline-alkaline tolerance in rice.The New phytologist · 2026Article
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- Differential condensation of FLO6 influences grain amylose synthesis in rice.Science advances · 2026Article
- Natural variation in BRN1 enhances nitrogen sensitivity to improve rice nitrogen use efficiency.Nature communications · 2026Article
- Two atypical resistance proteins confer plant resistance to Melanaphis sorghi in sorghum.Plant communications · 2026Article
- Energy-sensing kinase OsSnRK1b and its regulator OsCTK1 promote chilling-induced stomatal closure and cold tolerance.Nature communications · 2026Article
- A Non-Host Pathogen Elicitor Induces Blast Resistance Mediated by OsNAC78-Pir7b Module in Rice.Plant, cell & environment · 2026Article
- The C2H2-type zinc finger protein ZOS202 regulates leaf senescence in rice by targeting senescence-associated transcription factors and ROS homoeostasis.The Plant journal : for cell and molecular biology · 2026Article
- A Rare Allele of ST5 From Wild Rice Enhances Salt Tolerance in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Natural variation in OsMYB305 downregulating cytokinin-mediated inhibition of leaf senescence contributes to regional adaptation in rice.The New phytologist · 2026Article
- The transcription factor PdePHR1 increases phosphorus accumulation in poplar shoot and promotes root growth.BMC plant biology · 2026Article
- Precision Editing of Cis-Regulatory Elements Drives Crops Quantitative Traits Genetic Improvement: From Functional Dissection to Intelligent Design.Rice (New York, N.Y.) · 2026Review
417 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in investigations of plant signal transduction pathways related to hormones, metabolites and environmental challenges. In rice, protoplasts are commonly prepared from suspension cultured cells or etiolated seedlings, but only a few studies have explored the use of protoplasts from rice green tissue.
resultsHere, we report a simplified method for isolating protoplasts from normally cultivated young rice green tissue without the need for unnecessary chemicals and a vacuum device. Transfections of the generated protoplasts with plasmids of a wide range of sizes (4.5-13 kb) and co-transfections with multiple plasmids achieved impressively high efficiencies and allowed evaluations by 1) protein immunoblotting analysis, 2) subcellular localization assays, and 3) protein-protein interaction analysis by bimolecular fluorescence complementation (BiFC) and firefly luciferase complementation (FLC). Importantly, the rice green tissue protoplasts were photosynthetically active and sensitive to the retrograde plastid signaling inducer norflurazon (NF). Transient expression of the GFP-tagged light-related transcription factor OsGLK1 markedly upregulated transcript levels of the endogeneous photosynthetic genes OsLhcb1, OsLhcp, GADPH and RbcS, which were reduced to some extent by NF treatment in the rice green tissue protoplasts.
conclusionsWe show here a simplified and highly efficient transient gene expression system using photosynthetically active rice green tissue protoplasts and its broad applications in protein immunoblot, localization and protein-protein interaction assays. These rice green tissue protoplasts will be particularly useful in studies of light/chloroplast-related processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.