ArticleNature protocols2011
Using the Drosophila melanogaster D17-c3 cell culture system to study cell motility.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation of Drosophila collagen IV.Matrix biology : journal of the International Society for Matrix Biology · 2025Article
- Retinal Progenitor Cells Exhibit Cadherin-Dependent Chemotaxis across Transplantable Extracellular Matrix of In Vitro Developmental and Adult Models.Journal of tissue engineering and regenerative medicine · 2023Article
- TheMolecular biology of the cell · 2022Article
- Network-based prediction of polygenic disease genes involved in cell motility.BMC bioinformatics · 2019Article
- TheBiology open · 2019Article
- Generating and working with Drosophila cell cultures: Current challenges and opportunities.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- Ena/VASP processive elongation is modulated by avidity on actin filaments bundled by the filopodia cross-linker fascin.Molecular biology of the cell · 2019Article
- Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components.BMC developmental biology · 2019Article
- A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells.Journal of visualized experiments : JoVE · 2018Article
- CeFra-seq reveals broad asymmetric mRNA and noncoding RNA distribution profiles inRNA (New York, N.Y.) · 2018Article
- Comparative transcriptomic analysis of human and Drosophila extracellular vesicles.Scientific reports · 2016Article
- An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines.Molecular bioSystems · 2014Article
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Authors and funding
2 authors.
Funding
Abstract
Cultured Drosophila melanogaster S2 and S2R+ cell lines have become important tools for uncovering fundamental aspects of cell biology as well as for gene discovery. Despite their utility, these cell lines are nonmotile and cannot build polarized structures or cell-cell contacts. Here we outline a previously isolated, but uncharacterized, Drosophila cell line named Dm-D17-c3 (or D17). These cells spread and migrate in culture, form cell-cell junctions and are susceptible to RNA interference (RNAi). Using this protocol, we describe how investigators, upon receiving cells from the Bloomington stock center, can culture cells and prepare the necessary reagents to plate and image migrating D17 cells; they can then be used to examine intracellular dynamics or observe loss-of-function RNAi phenotypes using an in vitro scratch or wound healing assay. From first thawing frozen ampules of D17 cells, investigators can expect to begin assaying RNAi phenotypes in D17 cells within roughly 2-3 weeks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.