ArticleNature protocols2011
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed.
- Mechanisms of breast cancer dormancy in bone metastasis.Clinical & experimental metastasis · 2026Review
- Tie2 inhibition disrupts TMEM doorway function and reduces dissemination in pancreatic ductal adenocarcinoma.Journal of experimental & clinical cancer research : CR · 2026Article
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- Stem cell migration drives lung repair in living mice.Developmental cell · 2024Article
- Stabilized Window for Intravital Imaging of the Murine Pancreas.Journal of visualized experiments : JoVE · 2023Article
- Tracking Tumor Cell Dissemination from Lung Metastases Using Photoconversion.Journal of visualized experiments : JoVE · 2023Article
- Macrophages Promote Tumor Cell Extravasation across an Endothelial Barrier through Thin Membranous Connections.Cancers · 2023Article
- From mouse to human: Accessing the biochemistry of vision in vivo by two-photon excitation.Progress in retinal and eye research · 2023Review
- HyU: Hybrid Unmixing for longitudinal in vivo imaging of low signal-to-noise fluorescence.Nature methods · 2023Article
- Two-photon excitation fluorescence in ophthalmology: safety and improved imaging for functional diagnostics.Frontiers in medicine · 2023Review
- Intravital imaging to study cancer progression and metastasis.Nature reviews. Cancer · 2023Review
- Interleukin 4 Controls the Pro-Tumoral Role of Macrophages in Mammary Cancer Pulmonary Metastasis in Mice.Cancers · 2022Article
- Visualization of micro-agents and surroundings by real-time multicolor fluorescence microscopy.Scientific reports · 2022Article
- NR2F1 Is a Barrier to Dissemination of Early-Stage Breast Cancer Cells.Cancer research · 2022Article
- SWIP-a stabilized window for intravital imaging of the murine pancreas.Open biology · 2022Article
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- Primary tumor associated macrophages activate programs of invasion and dormancy in disseminating tumor cells.Nature communications · 2022Article
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Live tumor imaging shows macrophage induction and TMEM-mediated enrichment of cancer stem cells during metastatic dissemination.Nature communications · 2021Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Characterizing biological mechanisms dependent upon the interaction of many cell types in vivo requires both multiphoton microscope systems capable of expanding the number and types of fluorophores that can be imaged simultaneously while removing the wavelength and tunability restrictions of existing systems, and enhanced software for extracting critical cellular parameters from voluminous 4D data sets. We present a procedure for constructing a two-laser multiphoton microscope that extends the wavelength range of excitation light, expands the number of simultaneously usable fluorophores and markedly increases signal to noise via 'over-clocking' of detection. We also utilize a custom-written software plug-in that simplifies the quantitative tracking and analysis of 4D intravital image data. We begin by describing the optics, hardware, electronics and software required, and finally the use of the plug-in for analysis. We demonstrate the use of the setup and plug-in by presenting data collected via intravital imaging of a mouse model of breast cancer. The procedure may be completed in ∼24 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.