ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
Regulation of synaptic vesicle budding and dynamin function by an EHD ATPase.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed.
- A Quarter Century of EHD Protein Research: From Endosomal Recycling to Ciliopathies.Traffic (Copenhagen, Denmark) · 2026Review
- Dissecting membrane interfacial cellular processes: an in vitro reconstitution approach.The European physical journal. Special topics · 2024Review
- Dynamin2 functions as an accessory protein to reduce the rate of caveola internalization.The Journal of cell biology · 2023Article
- GTP-stimulated membrane fission by the N-BAR protein AMPH-1.Traffic (Copenhagen, Denmark) · 2023Article
- Coronin2A links actin-based endosomal processes to the EHD1 fission machinery.Molecular biology of the cell · 2022Article
- Synaptic Vesicle Recycling and the Endolysosomal System: A Reappraisal of Form and Function.Frontiers in synaptic neuroscience · 2022Review
- α-Synuclein in the Synaptic Vesicle Liquid Phase: Active Player or Passive Bystander?Frontiers in molecular biosciences · 2022Review
- Sorting nexin 17 (SNX17) links endosomal sorting to Eps15 homology domain protein 1 (EHD1)-mediated fission machinery.The Journal of biological chemistry · 2020Article
- Keeping in touch with the membrane; protein- and lipid-mediated confinement of caveolae to the cell surface.Biochemical Society transactions · 2020Review
- Energy and Dynamics of Caveolae Trafficking.Frontiers in cell and developmental biology · 2020Review
- Article
- Synaptic vesicle generation from activity-dependent bulk endosomes requires a dephosphorylation-dependent dynamin-syndapin interaction.Journal of neurochemistry · 2019Article
- The enigmatic endosome - sorting the ins and outs of endocytic trafficking.Journal of cell science · 2018Review
- Human Papillomavirus 16 Infection Induces VAP-Dependent Endosomal Tubulation.Journal of virology · 2018Article
- The trafficking protein, EHD2, positively regulates cardiac sarcolemmal KFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Role of the EHD Family of Endocytic Recycling Regulators for TCR Recycling and T Cell Function.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Retromer in Synaptic Function and Pathology.Frontiers in synaptic neuroscience · 2018Review
- Control of mitochondrial homeostasis by endocytic regulatory proteins.Journal of cell science · 2017Article
- EHD3 Protein Is Required for Tubular Recycling Endosome Stabilization, and an Asparagine-Glutamic Acid Residue Pair within Its Eps15 Homology (EH) Domain Dictates Its Selective Binding to NPF Peptides.The Journal of biological chemistry · 2016Article
- Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eps15 homology domain-containing proteins (EHDs) are conserved ATPases implicated in membrane remodeling. Recently, EHD1 was found to be enriched at synaptic release sites, suggesting a possible involvement in the trafficking of synaptic vesicles. We have investigated the role of an EHD1/3 ortholog (l-EHD) in the lamprey giant reticulospinal synapse. l-EHD was detected by immunogold at endocytic structures adjacent to release sites. In antibody microinjection experiments, perturbation of l-EHD inhibited synaptic vesicle endocytosis and caused accumulation of clathrin-coated pits with atypical, elongated necks. The necks were covered with helix-like material containing dynamin. To test whether l-EHD directly interferes with dynamin function, we used fluid-supported bilayers as in vitro assay. We found that l-EHD strongly inhibited vesicle budding induced by dynamin in the constant presence of GTP. l-EHD also inhibited dynamin-induced membrane tubulation in the presence of GTPγS, a phenomenon linked with dynamin helix assembly. Our in vivo results demonstrate the involvement of l-EHD in clathrin/dynamin-dependent synaptic vesicle budding. Based on our in vitro observations, we suggest that l-EHD acts to limit the formation of long, unproductive dynamin helices, thereby promoting vesicle budding.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.