ArticleJournal of clinical microbiology2011
Impact of genomic sequence variability on quantitative PCR assays for diagnosis of polyomavirus BK infection.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Interventions for BK virus infection in kidney transplant recipients.The Cochrane database of systematic reviews · 2024Pooled it
- Neutralization serotyping of BK polyomavirus infection in kidney transplant recipients.PLoS pathogens · 2012Trial
- BK Polyomavirus-associated nephropathy - diagnostic and treatment standard.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2025Review
- The impact of FDA-cleared molecular solutions for BK polyomavirus quantitation.Journal of clinical microbiology · 2025Review
- Diagnosing Polyomavirus Nephropathy Without a Biopsy: Validation of the Urinary Polyomavirus-Haufen Test in a Proof-of-Concept Study Including Uromodulin Knockout Mice.The Journal of infectious diseases · 2024Article
- The risk factors associated with post-transplantation BKPyV nephropathy and BKPyV DNAemia: a prospective study in kidney transplant recipients.BMC infectious diseases · 2024Article
- Review
- BK virus viral load: analysis of the requests received by the microbiology laboratory and clinical involvement of the issued results.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Calibration of BK Virus Nucleic Acid Amplification Testing to the 1st WHO International Standard for BK Virus.Journal of clinical microbiology · 2017Article
- A diverse virome in kidney transplant patients contains multiple viral subtypes with distinct polymorphisms.Scientific reports · 2016Article
- Reliable quantification of rhinovirus species C using real-time PCR.Journal of virological methods · 2016Article
- Sequence Variation in Amplification Target Genes and Standards Influences Interlaboratory Comparison of BK Virus DNA Load Measurement.Journal of clinical microbiology · 2015Article
- Comparative Evaluation of Three Nucleic Acid-Based Assays for BK Virus Quantification.Journal of clinical microbiology · 2015Article
- Phylogenetic reconstruction and polymorphism analysis of BK virus VP2 gene isolated from renal transplant recipients in China.Experimental and therapeutic medicine · 2015Article
- Toward standardization of BK virus monitoring: evaluation of the BK virus R-gene kit for quantification of BK viral load in urine, whole-blood, and plasma specimens.Journal of clinical microbiology · 2014Article
- BK polyomavirus genotypes represent distinct serotypes with distinct entry tropism.Journal of virology · 2013Article
- Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study.Jornal brasileiro de nefrologiaArticle
- Polyomavirus infection: can early diagnosis prevent development of associated allograft nephropathy?Jornal brasileiro de nefrologiaArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Knowledge of polyomavirus BK (BKV) genomic diversity has greatly expanded. The implications of BKV DNA sequence variation for the performance of molecular diagnostic assays is not well studied. We analyzed 184 publically available VP-1 sequences encompassing the BKV genomic region targeted by an in-house quantitative hydrolysis probe-based PCR assay. A perfect match with the PCR primers and probe was seen in 81 sequences. One Dun and 13 variant prototype oligonucleotides were synthesized as artificial targets to determine how they affected the performance of PCR. The sensitivity of detection of BKV in the PCR assay was a function of the viral genotype. Prototype 1 (BKV Dun) could be reliably detected at concentrations as low as 10 copies/μl. However, consistent detection of all BKV variants was possible only at concentrations of 10,000 copies/μl or higher. For BKV prototypes with 2 or more mismatches (representing genotype IV, genotype II, and genotype 1c strains), the calculated viral loads were 0.57 to 3.26% of the expected values. In conclusion, variant BKV strains lower the sensitivity of detection and may have a substantial effect on quantitation of the viral load. Physicians need to be cognizant of these effects when interpreting the results of quantitative PCR testing in transplant recipients, particularly if there is a discrepancy between the clinical impression and the measured viral load.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.