ArticleProteomics2011
Quantitative proteomics analysis of adsorbed plasma proteins classifies nanoparticles with different surface properties and size.
Article in Proteomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
41 citing papers in PubMed.
- The Impact of Serum Protein Adsorption on PEGylated NT3-BDNF Nanoparticles-Distribution, Protein Release, and Cytotoxicity in a Human Retinal Pigmented Epithelial Cell Model.Pharmaceutics · 2023Article
- Protein corona: Friend or foe? Co-opting serum proteins for nanoparticle delivery.Advanced drug delivery reviews · 2023Review
- Quantitative comparison of the protein corona of nanoparticles with different matrices.International journal of pharmaceutics: X · 2022Article
- Concentration and composition of the protein corona as a function of incubation time and serum concentration: an automated approach to the protein corona.Analytical and bioanalytical chemistry · 2022Article
- Understanding the Significance of Sample Preparation in Studies of the Nanoparticle Metabolite Corona.ACS measurement science au · 2022Article
- Effects of Acute Exposure to Polystyrene Nanoplastics on the Channel Catfish Larvae: Insights From Energy Metabolism and Transcriptomic Analysis.Frontiers in physiology · 2022Article
- Interaction of Carbon Dots from Grilled Spanish Mackerel with Human Serum Albumin, γ-Globulin and Fibrinogen.Foods (Basel, Switzerland) · 2021Article
- Environmental dimensions of the protein corona.Nature nanotechnology · 2021Review
- Nanoparticle delivery system, highly active antiretroviral therapy, and testicular morphology: The role of stereology.Pharmacology research & perspectives · 2021Review
- GMMA-Based Vaccines: The Known and The Unknown.Frontiers in immunology · 2021Review
- SWATH-MS Protocols in Human Diseases.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Micro- and nanoplastics - current state of knowledge with the focus on oral uptake and toxicity.Nanoscale advances · 2020Review
- A Protein Corona Adsorbed to a Bacterial Magnetosome Affects Its Cellular Uptake.International journal of nanomedicine · 2020Article
- A proteome-wide assessment of the oxidative stress paradigm for metal and metal-oxide nanomaterials in human macrophages.NanoImpact · 2020Article
- Best practice in reporting corona studies: Minimum information about Nanomaterial Biocorona Experiments (MINBE).Nano today · 2019Article
- All that is silver is not toxic: silver ion and particle kinetics reveals the role of silver ion aging and dosimetry on the toxicity of silver nanoparticles.Particle and fibre toxicology · 2018Article
- Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials.Analytical and bioanalytical chemistry · 2018Review
- Current Application of Capillary Electrophoresis in Nanomaterial Characterisation and Its Potential to Characterise the Protein and Small Molecule Corona.Nanomaterials (Basel, Switzerland) · 2018Review
- The Chameleon Effect: Characterization Challenges Due to the Variability of Nanoparticles and Their Surfaces.Frontiers in chemistry · 2018Article
- NanoEHS beyond Toxicity - Focusing on Biocorona.Environmental science. Nano · 2017Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
Nanoparticle biological activity, biocompatibility and fate can be directly affected by layers of readily adsorbed host proteins in biofluids. Here, we report a study on the interactions between human blood plasma proteins and nanoparticles with a controlled systematic variation of properties using (18)O-labeling and LC-MS-based quantitative proteomics. We developed a novel protocol to both simplify isolation of nanoparticle bound proteins and improve reproducibility. LC-MS analysis identified and quantified 88 human plasma proteins associated with polystyrene nanoparticles consisting of three different surface chemistries and two sizes, as well as, for four different exposure times (for a total of 24 different samples). Quantitative comparison of relative protein abundances was achieved by spiking an (18)O-labeled "universal" reference into each individually processed unlabeled sample as an internal standard, enabling simultaneous application of both label-free and isotopic labeling quantification across the entire sample set. Clustering analysis of the quantitative proteomics data resulted in distinctive patterns that classified the nanoparticles based on their surface properties and size. In addition, temporal data indicated that the formation of the stable protein corona was at equilibrium within 5 min. The comprehensive quantitative proteomics results obtained in this study provide rich data for computational modeling and have potential implications towards predicting nanoparticle biocompatibility.
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Registered trials
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